Flash photolysis split beam spectrophotometer

Optics: measuring and testing – Range or remote distance finding – With photodetection

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23230B, 23253R, 356 85, 356 95, G01J 342

Patent

active

040239055

ABSTRACT:
Method and apparatus for providing flash photolysis of CO-inhibited cytochrome oxidase in a first portion of a sample exposed to an oxygenating agent and optically comparing the result with a non-photolyzed second portion of the sample or with a previously photolyzed portion of the sample. The sample is contained in a tubular transparent chamber located in the optical scanning path of a split-beam spectrophotometer having respective split-beam light guide channels traversing two spaced portions of the tubular sample chamber. Two corresponding photolyzing light guide channel assemblies are provided perpendicular to the scanning light guide channels, coplanar therewith, with a laser adjustably positioned to selectively flash-illuminate either of the two transverse photolyzing light guide channels optically communicating with the sample chamber. The technique may be employed either with frozen samples or with samples in the liquid state, with the viscosity suitably increased and with a constriction between the two portions of the sample chamber to inhibit mixing.

REFERENCES:
patent: 3811777 (1974-05-01), Chance
patent: 3830222 (1974-08-01), Chance
Boxall et al., Proc. R. Soc. Lond.; vol. 328, No. 1575, 1972, pp. 515-527.

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