Flap endonuclease mutants

Details Flap endonuclease mutants Flap endonuclease mutants

2005-01-21

2010-12-07

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S019000, C435S320100, C435S069100, C435S252300, C435S325000, C530S350000, C536S023200

Reexamination Certificate

active

07846704

ABSTRACT:
A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.

REFERENCES:
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Seffernick et al., J. Bacteriol. 183(8):2405-2410, 2001.
Eriko Matsui, et al, “Aromatic Residues Located Close to the Active Center are Essential for the Catalytic Reaction of Flap Endonuclease-1 From Hyperthermophilic ArchaeonPyrococcus Horikoshii”, The Journal of Biological Chemistry, vol. 279, No. 16, 2004, pp. 16687-16696.
Tony M. Hsu, et al, “Genotyping Single-Nucleotide Polymorphisms by the Invader Assay With Dual-Color Fluorescence Polarization Detection”, Clinical Chemistry, vol. 47, No. 8, 2001, p. 1373-1377.
Junzhuan Qiu, et al, “Interaction Interface of Human Flap Endonuclease-1 With its DNA Substrates”, The Journal of Biological Chemistry, vol. 279, No. 23, 2004, pp. 24394-24402.
Binghui Shen, et al, “Essential Amino Acids for Substrate Binding and Catalysis of Human Flap Endonuclease 1”, The Journal of Biological Chemistry, vol. 271, No. 16, 1996, pp. 9173-9176.

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