Fish growth hormones

Details Fish growth hormones Fish growth hormones

2000-04-14

2002-08-06

Nguyen, Dave T. (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S024310, C435S091200

Reexamination Certificate

active

06429305

ABSTRACT:

BACKGROUND OF THE INVENTION
Farmed fish, such as ayu or grouper, are commercially valuable crops. A means of increasing the growth rate or size of fish crops is therefore commercially useful.
SUMMARY OF THE INVENTION
The invention is based on the discovery of three new fish growth hormone genes (from Plecoglossus altivelis, Epinephelus awoara, and Danio rerio) that can be used to increase fish crop production. For example, each of the new fish growth hormone genes can be used to generate transgenic fish expressing a fish growth hormone of the invention. These fish will exhibit greater growth rates and body sizes than the parent fish from which the transgenic fish was derived. Alternatively, the growth hormones can be recombinantly produced and introduced into the fish diet (see, e.g., Ben-Atia et al., Gen. Compar. Endocrinol. 113:155-164, 1999).
In addition, the growth hormone polypeptides and fragments thereof can be used to generate antibodies that specifically bind to a growth hormone of the invention. These antibodies can be used to, e.g., detect fish pituitary tissue in a sample. Other fragments, such as the signal sequence, can be fused to heterologous proteins to render them secretable. The growth hormone cDNA and fragments thereof can be used to screen DNA libraries (e.g., genomic or cDNA libraries) to isolate other related genes and the growth hormones which they encode.
The sequences of the new fish growth hormones are given below:
Plecoglossus altivelis (common name: ayu)
GGCACGAGCA GAGACCAGCG ATTCACCCAG AGTTCTCTAC TGACGACATC AGATACGACA

AGGACAGAAT GGGGCAAGTG CTTCTCCTGG TGACCCTCCT GCTGGTCAGT GACCTGGTCA
         M   G  Q   V  L  L  L   V  T   L   L  L  V  S   D L  V

GATCTGCATC CGGCTCAGAG AACCAGCGTC TCTTCAGCAT CGCTGTCAAT CGTGCTCAAC
R S   A  S   G  S   E  N  Q  R   L  F   S   I  A  V  N   R A  Q

ACCTGCACCT GCTGGCCCAG AAGATGTTCA ACGACTTTGA GGGAAACCTC TCTCCAGATG
H L   H  L   L  A   Q  K  M  F   N  D   F   E  G  N  L   S P  D

ATCGAAGGCA GATGAATAAG ATCTTCCTGC AGGACTTCTG TAACTCAGAC TCCATCATCA
D R   R  Q   M  N   K  I  F  L   Q  D   F   C  N  S  D   S I  I

GCCCCGTGGA CAAGCACGAG ACTCAGAAGA GCTCGGTGCA GAAGTTGCTC CACATCTCGT
S P   V  D   K  H   E  T  Q  K   S  S   V   Q  K  L  L   H I  S

TCCGTCTGGT GGAGTCGTGG GAGTACCCCA GCCAACCTCT CAGCACCTCA CTGACCCTCA
F R   L  V   E  S   W  E  Y  P   S  Q   A   L  S  S  S   L S  L

GTCGCTTCAG TGAGATCCCT CTGAAACTCA CCGACCTGAA GCTGGGCATC GACACCATCC
S R   F  S   E  I   P  L  K  L   T  D   L   K  L  G  I   D T  I

TCAGGGGTAC CCAAGATGGG CTCCTCAGCC TGGAGGATAA TGAGGCCCAG CAGCTGCCCC
L R   G  T   Q  D   G  L  L  S   L  E   D   N  E  A  Q   Q L  P

CCTATGAGAA TTACTACCAG AACCTCGGGG GTGACGACAA CATCCACAGG AGCTACGAGC
P Y   E  N   Y  Y   Q  N  L  G   G  D   D   N  I  H  R   S Y  E

TGCTAGCCTG CTTCAAGAAA GACATGCACA AGGTGGAAAC GTACCTGACT GTTGCAAAGT
L L   A  C   F  K   K  D  M  H   K  V   E   T  Y  L  T   V A  K

GTCGTCGATC CCTCGAAGCC AACTGTACCC TGTAGAACCA CGCCTGACAG ACAGAACCAA
C R   R  S   L  E   A  N  C  T   L  * (SEQ ID NO:2)

TCTATCTCAG ATCAACTTAG CCCTGGACTG TGTCACATTT AATATCCTCC CCCCTCCATA

TTCCATACTT TCTGCTTTGG AATGAAACTA TATAAATAAA CCAACGTGTA TTTACAAAAA

GTATAACTAT ATATTTTGTT AAGGTTCGTT TGPAAGCAGA AGGAATGGAA TCTCTCAAGA

GGACAGAGAG GGAAGGATGT CGCAATCCAA CGTTGTTTTG TTTTGAAGTG TAGGTTTGTT

CTGAGACACC AGGCTCATGG TTTTCCATCT TACCAACAAC AGCACTTTCT ATACGTCTGT

GTCTTTTCCT ATTAGGAGAG TCTGATTAAG GATATTTCAG CCAGATAAGT GTTTCTAATG

GAGGTGAATT AGTTTGTTAC TCGCAAACGA TTCAAGATAC AATTCATATT GGTGCTCATT

TTCATTAGAA ATCAAAACAT ACTAACTCAC AGAAGATGTA TGTTTTAATA ACAGTTTATG

TCCATTTGGG CACATTGATA ATGATTGCCT GTCGATGAAA AGACTCCCAT ATGTATTTGT

GTTTTGTTTT TTAAGTATTA AGAGATGTTT TGTCATGAAG ACATCTTTCT ACTTGATTCA

TTTGCCTCTC CTTTTTACTT TACGTGAAAT GTGTCATTGT TTTTAATTTC T
AATAAA
GCT

GTTGTTTATT GCAAAAAAAA AAAAAAAAAA (SEQ ID NO:1)
The leader peptide sequence (SEQ ID NO:3) is shown in bold, and the polyA signal is underlined. The nucleotide sequence between the initiation codon and the polyA signal, excluding the initiation codon and the polyA signal, is designated SEQ ID NO:4.
Epinephelus awoara (common name: yellow grouper)
ACGAGCTCAG ACCTGATCCA CCAGAGCCAG ACCTGATCCC CCAGAGCCAG ACCTAATCCC

AGACCAGCCA TGGACCGAGT CGTCCTCCTG CTGTCAGTAG TGTCTCTGGG CGTTTCCTCT
         M   D  R   V  V  L  L   L  S   V   V  S  L  G   V G  S

CAGCCAATCA CAGACGGCCA GCGACTGTTC TCCATCGCTG TCAGCAGAGT TCAACATCTC
Q P   I  T   D  G   Q  R  L  F   S  I   A   V  S  R  V   Q R  L

CACCTGCTTG CTCAGAGACT CTTCTCCGAC TTTGAGAGCA GTCTGCAGAC AGAGGAGCAG
H L   L  A   Q  R   L  F  S  D   F  E   S   S  L  Q  T   E E  Q

CGACAGCTCA ACAAGATCTT CCTGCAGGAC TTTTGTAACT CTGATTACAT CATCAGCCCC
R Q   L  N   K  I   F  L  Q  D   F  C   N   S  D  Y  I   I S  P

ATTGACAAGC ATGAGACGCA GCGCAGCTCC GTGTTGAAGC TGTTGTCGAT CTCCTATCGG
I D   K  H   E  T   Q  R  S  S   V  L   K&ems

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