Fingerprinting using single specific primers in low stringency p

Details Fingerprinting using single specific primers in low stringency p Fingerprinting using single specific primers in low stringency p

1995-03-20

1997-02-04

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 422 681, 536 231, C12Q 168, C12P 1934, C07H 2104

Patent

active

055996744

ABSTRACT:
A straightforward method to characterize a nucleotide sequence of a defined DNA region is described. A purified segment representing the defined region is subjected to repeated cycles of amplification (PCR) using a single primer complementary to a portion of the segment under conditions of low stringency and high polymerase and primer concentrations. PCR under these conditions results in a mixture of amplified DNA molecules the composition of which is characteristic of the DNA segment assessed. Determination of the composition of the mixture can be obtained by subjecting the mixture to standard chromatographic or electrophoretic techniques to obtain a pattern of fragments which represents a "signature" of the nucleotide sequence.

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