Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-02-09
1999-09-28
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4353201, 435820, 530350, 530811, 536 237, C07K 14195, C12N 1531, C12N 1574, G01N 3353
Patent
active
059587067
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to protein-bound particles and a production method thereof, as well as a novel protein, gene and so on, and a method for assaying a target substance by the use of the protein-bound magnetic particles.
BACKGROUND ART
Proteins having biological activities such as enzymes and antibodies immobilized on magnetic particles can be led by magnetic means. Therefore, they can be led to local positions at which it has been hitherto difficult for them to reach. Further, they can be collected and separated by means of a magnetic force. Thus they are expected to be utilized in various industries including the fields of medicine and fermentation.
In the prior art, for example, immobilization of biologically active substances to magnetic particles is disclosed in Japanese Patent Publication (KOKOKU) No. 6-12994. Namely, magnetic particles are separated from a magnetic bacterium by an alkaline treatment, and they are further treated with .UPSILON.-aminopropyltriethoxysilane or glutaraldehyde, and to them a biologically active substance is chemically immobilized. A method is also known, in which magnetic particles are separated by an enzyme treatment from a magnetic bacterium in a state of being covered with an organic thin membrane comprising lipid, and a protein is immobilized thereto after a treatment with glutaraldehyde. A method is also known in which a biologically active substance is immobilized on magnetic particles by a chemical binding method using SPDP (Japanese Pre-examination Patent Publication (KOKAI) No. 5-209884).
Further, methods for measuring antigens or antibodies have been proposed, in which an antigen-antibody reaction is allowed to occur by way of the magnetic particles to which a biologically active substance has been chemically immobilized by the aforementioned methods (Japanese Pre-examination Patent Publication (KOKAI) Nos. 4-285857, 5-209884, and 5-99926).
However, in any of the foregoings, it is necessary for a protein such as an enzyme of an antibody, etc. to be chemically bound, to magnetic particles. Thus poblems have arisen: a long time is required for the immobilization treatment; the biological activity of the protein is impaired; the amount of immobilized protein is greatly dispersed from one lot to another, and its biological activity is also greatly dispersed from one lot to another; and the immobilized protein inevitably becomes expensive because the protein to be used for immobilization is generally expensive.
Further, the aforementioned method in which labeled antibodies have been bound to magnetic particles before use makes it necessary that, in advance, a labeling substance is chemically bound or physically adsorbed to the antibodies. Because of this, the antibodies may lose its activity, or lose its fraction due to the treatment. Furthermore, the amount and binding mode of the labeling substance immobilized to the magnetic particles may vary, causing a variation in measurement data, which may, in turn, lead to an increased cost.
Accordingly, an object of this invention is to overcome the problems accompanying the conventional techniques in which an enzyme, antibody or labeling compound is immobilized to magnetic particles by a chemical method, and to provide a method enabling a high sensitivity measurement of a target substance, and magnetic particles to be used in that method.
DISCLOSURE OF INVENTION
The first aspect of this invention consists in providing a useful protein-bound magnetic particle which comprises: a magnetic particle which is produced in the cell of a magnetic bacterium, and a hybrid protein bound to an organic membrane covering the magnetic particle; said hybrid protein comprising a polypeptide chain containing at least a membrane-bound portion of a membrane protein which is originally produced in a state of being bound to the organic membrane and at least one other protein which is biologically bound to said polypeptide.
BRIEF DESCRIPTION OF DRAWINGS
FIG. 1 is a chart representing hydrophilic-hydrophobic reg
REFERENCES:
Nakamura, et al: Iron-Regulated Expression and Membrane Localization of the MagA Protein in Magnetospirillum sp. Strain AMB-1.sup.1, J. Biochem., vol. 118, No. 1, 1995, 23-27 (1995).
Nakamura, et al: An Iron-Regulated Gene, magA, Encoding and Iron Transport protein of Magnetospirillum sp. Strain AMB-1*, The JOuranl of Biological Chemistry, vol. 270, No. 47, pp. 28392-28396.
Kamiya Shinji
Matsunaga Tadashi
Namba Kenryo
Degen Nancy
Matsunaga Tadashi
Schwartzman Robert
TDK Corporation
LandOfFree
Fine magnetic particles containing useful proteins bound thereto does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fine magnetic particles containing useful proteins bound thereto, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fine magnetic particles containing useful proteins bound thereto will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-702091