Filamentous fungi functional replicating extrachromosomal elemen

Details Filamentous fungi functional replicating extrachromosomal elemen Filamentous fungi functional replicating extrachromosomal elemen

1983-01-24

1984-12-04

Wiseman, Thomas

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 70, 435 91, 435254, 435317, 935 24, 935 68, 935 72, 935 73, C12N 1500, C12N 114, C12N 100, C12P 2100, C12P 2102, C12P 1934

Patent

active

044865333

ABSTRACT:
A functional extrachromosomal element capable of replication in filamentous fungi is provided. The extrachromosomal element employs (1) a mitochondrial replicating element or (2) a lower organism replication sequence recognized by the fungus, in combination with foreign DNA to provide replication, transcription, and translation of foreign regulatory elements and genes. The extrachromosomal element is exemplified by a mitochondrial replicating system from Neurospora.
The cell strain E. coli HB101 containing the plasmid pALS-1-1 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39157.
The cell strain E. coli HB101 containing the plasmid pALS-2 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39158.

REFERENCES:
Lambowitz et al.: J. Bacteriology 108, 1087 (1971).
Stohl et al.: Nucl. Acids Res. 10, 1439 (1982).
Collins et al.: Cell 443 (1981).
Stahl et al.: Molec. Gen. Genet. 178, 639 (1980).
Zakian: Proc. Natl. Acad. Sci. USA 78, 3128 (1981).

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