Optical: systems and elements – Compound lens system – Microscope
Patent
1998-04-08
2000-04-25
Nguyen, Thong
Optical: systems and elements
Compound lens system
Microscope
359368, 359385, G02B 2106, G02B 2100
Patent
active
06055097&
ABSTRACT:
In an improved optical microscope for observing a luminescent specimen, the specimen is excited by a single, on axis standing wavefield or multiple superposed series of standing wave fields. Then an image of the specimen is recorded and displayed. This specimen can be incrementally moved and additional images can be recorded and processed. Images of the specimen recorded when there are nodes or antinodes at the focal plane of the microscope can be combined by image processing to produce an improved image or set of images of the specimen. Also disclosed are improved standing wave microscopes having a phase conjugator, a transmitted light source, feedback stabilization, an extended light source for field synthesis or a beam contractor. A multiple wavelength light source can be used to view a specimen marked with multiple luminescent dyes.
REFERENCES:
patent: 4311358 (1982-01-01), Gibbons et al.
patent: 4621911 (1986-11-01), Lanni et al.
patent: 4917462 (1990-04-01), Lewis et al.
patent: 5018865 (1991-05-01), Ferrell et al.
patent: 5035476 (1991-07-01), Ellis et al.
patent: 5073018 (1991-12-01), Kino et al.
patent: 5225923 (1993-07-01), Montagu
patent: 5386112 (1995-01-01), Dixon
patent: 5394268 (1995-02-01), Lanni et al.
Gustafsson et al., Sevenfold improvement of axial resolution in 3D widefield microscopy using two objective lens, Sep. 1995, SPIE vol.2412, pp. 147-156.
"Standing-Wave Fluorescence Microscopy" by Frederick Lanni, Applications of Fluorescence in the Biomedical Sciences, D.L. Taylor, A.S. Waggoner, R.F. Murphy, F. Lanni and R.R. Birge, eds., Alan R. Liss, Inc., New York, New York 1986, pp. 505-521.
Proceedings of the SPIE: Los Alamos Conference On Optics, vol. 190, 23 May 1979, Los Alamos, NM, US, P.A. Temple, "Improved Dark-field-like Surface Inspection Technique using Total Internal Reflection".
Soviet Patents Abstracts, Section EI, Week 9218, Derwent Publications Ltd., London, GB; Class S05, p.4, AN 9248427 & SU A,1,374,922 (As Sibe Bio-Phys 30 Jul. 1991.
Applied Optics, vol. 20, No. 15, 1 Aug., 1981, New York, U.S. pp. 2656-2664, PA Temple, "Total Internal Reflection Microscopy: A Surface inspection Technique.".
"Feedback -Stabilized Focal Plane Control For Light Microscopes" by Frederick Lanni; Rev. Sci. Instrum. 64(6), pp. 1474-1477, Hun. 1993
Lanni et al., "Excitation Field Synthesis As a Means for Obtaining Enhanced Axial Resolution In Fluorescence Microsopes, " Bioimaging 1 (1993), pp. 187-196.
Bailey Brent
Lanni Frederick
Taylor D. Lansing
Carnegie Mellon University
Nguyen Thong
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