Field synthesis and optical subsectioning for standing wave micr

Details Field synthesis and optical subsectioning for standing wave micr Field synthesis and optical subsectioning for standing wave micr

1993-02-05

1995-02-28

LaRoche, Eugene R.

Optical: systems and elements

Compound lens system

Microscope

359368, G02B 2106

Patent

active

053942680

ABSTRACT:
In an improved optical microscope for observing a luminescent specimen, the specimen is excited by a time-multiplexed series of standing wave fields. Then an image of the specimen is recorded and displayed. This specimen can be incrementally moved and additional images can be created and combined. Images of the specimen can also be created when there are nodes or antinodes at the focal plane of the microscope. These images can also be combined to produce an improved image of the specimen.

REFERENCES:
patent: 4311358 (1982-01-01), Gibbons
patent: 4621911 (1986-11-01), Lanni et al.
"Standing-Wave Fluorescence Microscopy" by Frederick Lanni, Applications of Fluorescence in the Biomedical Sciences, D. L. Taylor, A. S. Waggoner, R. F. Murphy, F. Lanni and R. R. Birge, eds., Alan R. Liss, Inc., New York, N.Y. 1986, pp. 505-521.
Proceedings Of The Spie: Los Alamos Conference On Optics, vol. 190, 23 May 1979, Los Alamos, N. Mex. US, P. A. Temple, "Improved Dark-field-like Surface Inspection Technique using Total Internal Reflection".
Soviet Patents Abstracts, Section EI, Week 9218, Derwent Publications Ltd., London, GB; Class S03, p. 4, AN 92148427 & SU,A 1,374,922 (As Sibe Bio-Phys) 30 Jul. 1991.
Applied Optics, vol. 20, No. 15, 1 Aug. 1981, New York, U.S., pp. 2656-2664, P. A. Temple, "Total Internal Reflection Microscopy: A Surface Inspection Technique".

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