Optical: systems and elements – Compound lens system – Microscope
Patent
1995-02-23
1998-09-01
Nguyen, Thong
Optical: systems and elements
Compound lens system
Microscope
359368, 359385, G02B 2106, G02B 2100
Patent
active
058018816
ABSTRACT:
In an improved optical microscope for observing a luminescent specimen, the specimen is excited by a single, on axis or multiple superposed series of standing wave fields. Then an image of the specimen is recorded and displayed. This specimen can be incrementally moved and additional images can be recorded and processed. Images of the specimen recorded when there are nodes or antinodes at the focal plane of the microscope can be combined by image processing to produce an improved image or set of images of the specimen. Also disclosed are improved standing wave microscopes having a phase conjugator, a transmitted light source, feedback stabilization, an extended light source for field synthesis or a beam contractor. A multiple wavelength light source can be used to view a specimen marked with multiple luminescent dyes.
REFERENCES:
patent: 4311358 (1982-01-01), Gibbons
patent: 4621911 (1986-11-01), Lanni et al.
patent: 4917462 (1990-04-01), Lewis et al.
patent: 5073018 (1991-12-01), Kino et al.
patent: 5225923 (1993-07-01), Montagu
patent: 5386112 (1995-01-01), Dixon
Proceedings Of The Spie: Los Alamos Conference On Optics, vol. 190, 23 May 1979, Los Alamos, NM. US, P. A. Temple, "Improved Dark-field-like Surface Inspection Technique using Total Internal Reflection".
Soviet Patents Abstracts, Section EI, Week 9218, Derwent Publications Ltd., London, GB; Class S03, p. 4, AN 92148427 & SU,A 1,374,922 (As Sibe Bio-Phys) 30 Jul. 1991.
Applied Optics, vol. 20, No. 15, 1 Aug., 1981, New York, U.S., pp. 2656-2664, P. A. Temple, "Total Internal Reflection Microscopy: A Surface Inspection Technique".
"Standing-Wave Fluorescence Microscopy" by Frederick Lanni, Applications of Fluorescence in the Biomedical Sciences, D. L. Taylor, A. S. Waggoner, R. F. Murphy, F. Lanni and R. R. Birge, eds., Alan R. Liss, Inc., New York, New York,1986, pp. 505-521.
F. Lanni "Feedback-stabilized focal plane control for light microscopes", Rev. Sci. Instrum. 64 (6), pp. 1474-1477, Jun., 1993.
Gustafsson et al., Sevenfold improvement of axial resolution in 3D widefield microscopy using two objective lens, Sep. 1995, SPIE vol. 2412, pp. 147-156.
Bailey Brent
Lanni Frederick
Taylor D. Lansing
Carnegie Mellon University
Nguyen Thong
LandOfFree
Field synthesis and optical subsectioning for standing wave micr does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Field synthesis and optical subsectioning for standing wave micr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Field synthesis and optical subsectioning for standing wave micr will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-276040