Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-08-06
2000-01-11
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435177, 435179, C12P 1902, C12N 1102, C12N 1112
Patent
active
060134917
ABSTRACT:
A biocatalyst for converting sucrose solutions to glucose-fructose solutions in a fluidized bed reactor is provided containing yeast adhered to a fibrous cellulose support. A method of making the biocatalyst is carried out by mixing yeast with water to form a yeast paste; mixing the yeast paste with a fibrous cellulose support to form a fiber-yeast mixture; treating the fiber-yeast mixture with a fixing agent; and drying the treated fiber-yeast mixture at a temperature not exceeding 70.degree. C. The fibrous cellulose support preferably contains fibers ranging from approximately 0.5 cm to 3.0 cm in length obtained by processing of sugar cane bagasse or ground corn cobs. Preferably, the fixing agent is glutaraldehyde, cellulose triacetate, diethylaminoethyl-cellulose or a reaction product of polyethylenimine with 1,2-dichloroethane, and the yeast is Saccharomyces cerevisiae. A method for producing a glucose-fructose solution from a sucrose solution is provided containing the steps of passing the sucrose solution over the biocatalyst in a fluidized bed reactor and recovering the glucose-fructose solution.
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Dolce Carla J.
Naff David M.
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