Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1990-01-05
1992-12-08
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530413, C07K 320, C07K 1512
Patent
active
051699303
ABSTRACT:
The present invention provides a substantially pure integrin-type receptor characterized in that it consists of an .alpha..sub.v and a .beta..sub.1 subunit. The .alpha..sub.v .beta..sub.1 integrin binds to fibronectin and GRGDSPK but does not bind to vitronectin. The .alpha..sub.v .beta..sub.1 integrin can be used to determine the presence of a .alpha..sub.v .beta..sub.1 ligand and to develop adhesion peptides specific for the various integrins. The presence of the .alpha..sub.v .beta..sub.1 integrin can be used to assess ability of cells to adhere to fibronectin.
REFERENCES:
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Dedhar et al., Isolation of a Novel Integrin Receptor Mediating Arg-Gly-Asp-Directed Cell Adhesion to Fibronectin and Type i Collagen from Human Neuroblastoma Cells Association of a Novel Beta-1-Related Subunit with Alpha-v, J. Cell. Biol., 110(6): 2185-2194 (1990) (Abstract only).
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Freed et al., A Novel Integrin B Subunit is Associated with the Vitronectin Receptor a Subunit (a.sub.v) in a Human Osteosarcoma Cell Line and is a Substrate for Protein Kinase C, The EMBO Journal 8: 2955-2965 (1989).
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Giancotti Filippo G.
Ruoslahti Erkki I.
Tarone Guido
Vogel Bruce E.
La Jolla Cancer Research Foundation
Russel Jeffrey E.
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