Fibronectin purification vector

Details Fibronectin purification vector Fibronectin purification vector

1991-01-03

1994-08-30

Wax, Robert C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435273, 4353201, 435804, 435814, 530415, 530417, 536 231, 536 242, C12N 1511, C12N 1566, C07K 312, C07K 318

Patent

active

053427629

ABSTRACT:
Recombinant protein purification vectors and methods for their use are disclosed. The vectors contain a DNA sequence coding for a gelatin binding region of fibronectin. The vectors express a foreign DNA sequence of interest fused to the fibronectin portion. Secretion signals on the fused product assist the product in being secreted from a production cell. The product can then be purified on a gelatin containing affinity column and digested with a protease such as trypsin to cleave the desired protein from the gelatin binding region. The vectors can also be designed to code for factor XIIIa cross liking sites and to have a chemically reactive cysteine residue.

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