Fibroblast growth factor gene transformed hybridoma and enhanced

Details Fibroblast growth factor gene transformed hybridoma and enhanced Fibroblast growth factor gene transformed hybridoma and enhanced

1991-05-29

1993-05-25

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351723, 435 691, 435 694, 935 55, C12N 512

Patent

active

052139808

ABSTRACT:
Disclosed are (1) a hybridoma carrying a vector for expressing a fibroblast growth factor (FGF) protein gene; (2) a method for producing the hybridoma of (1) which comprises transforming a hybridoma with the vector for expressing the FGF protein gene; and (3) a method for producing a biologically active substance which comprises cultivating in a culture medium the hybridoma obtained by the method of (2) using a hybridoma producing a biologically active substance other than the FGF protein, producing the FGF protein and producing and accumulating the biologically active substance in a culture, and recovering the biologically active substance, whereby the biologically active substance can be efficiently produced and recovered using the serum-free medium, which is advantageous for industrial production and very useful for an improvement in the breeding of the hybridoma.

REFERENCES:
Kovar, J. "Hybridoma Cultivation in Defined Serum-Free Media: Growth Supporting Substances . . . " Folia Biologica (Praha) 32:304-310, 1986.
Pendse & Bailey, "Effects of Growth Factors on Cell Proliferation And Monoclonal Antibody Production of Batch Hybridoma Cultures", Biotechnology Letters, vol. 12, No. 7, 487-492 (1990).
Sasada et al. Molecular & Cellular Biology 8 588 (1988).
Harlow et al. from Antibodies, A Laboratory Manual, Cold Spring Harbor, pp. 142, 241, 245-253, 1988.
Gospodarowicz, D. Nature, 249:123 (1974).
Genot, et al. Cellular Immunology, 122:424 (1989).
Sasada, et al. Molecular and Cellular Biology, 8:588 (1988).

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