Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1994-06-10
1996-11-19
Walsh, Stephen G.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
514 8, 424 945, 530350, 530370, 530399, 530402, 530409, A61K 3818, A61K 3845, C07K 1450, C07K 14415
Patent
active
055762883
ABSTRACT:
The invention provides a conjugate comprising FGF or other polypeptide reactive with an FGF receptor, and a cytotoxic agent. The cytotoxic agent can be a ribosome-inactivating protein (RIP), such as saporin, although other cytotoxic agents can also be advantageously used. The cytotoxic agent can be attached to FGF through a chemical bond, or the composition can be prepared as a chimera using techniques of recombinant DNA. The conjugate can be used to treat FGF-mediated pathophysiological conditions by specifically targeting cells having FGF receptors and inhibiting proliferation of or causing death of such cells. Additionally, the conjugate can be used to target cytotoxic agents into cells having FGF receptors to inhibit the proliferation of such cells. The conjugate can be purified on an immobilized-heparin column.
REFERENCES:
patent: 4468382 (1984-08-01), Bacha et al.
patent: 4962188 (1990-10-01), Frankel
patent: 5087616 (1992-02-01), Meyers et al.
patent: 5116753 (1992-05-01), Beattie et al.
patent: 5122368 (1992-06-01), Greenfield et al.
patent: 5155214 (1992-10-01), Baird et al.
patent: 5169933 (1992-12-01), Anderson et al.
patent: 5191067 (1993-03-01), Lappi et al.
Huang et al "Association of Bovine Brain-Derived Growth Factor Receptor . . . " J. Biol. Chem. 21:9568-9571 (Jul. 1986).
Imamura et al "Purification of Basic FGF Receptors from Rat Brain" Biochem Biophys. Res. Comm. 155(2):583-590 (Sep. 1988).
Baird et al., "Angiogenic factor in human ocular fluid," The Lancet, Sep. 7, 1985, p. 563.
Baird et al., "Fibroblast growth factors," Brit. Med. Bull., 45(2):438-452 (1989).
Barbieri et al., "Ribosome-inactivating proteins from plants: Properties and possible uses," Cancer Surveys, 1(3):489-520 (1982).
Gospodarowicz, D., "Isolation and characterization of acidic and basic fibroblast growth factor," Methods in Enzymology, 147:106-119 (1987).
Montecucchi et al., "N-terminal sequence of some ribosome-inactivating proteins," Int. J. Peptide Protein Res., 33:263-267 (1989).
Lappi et al., "Characterization of a saponaria officinalis seed ribosome-inactivating protein: Immunoreactivity and sequence homologies," Biochem. and Biophys. Res. Comm., 129(3):934-942 (1985).
Stirpe et al., "Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree)", Biochem. J., 216:617-625 (1983).
Blakey, et al., "Comparison of the pharmacokinetics and hepatotoxic effects of saporin and ricin A-chain immunotoxins on murine liver parenchymal cells," Cancer Research, 48(24pt1):7072-7078 (1988).
Akiyama et al., Verapamil enhances the toxicity of conjugates of epidermal growth factor with Pseudomonas exotoxin and antitransterrin receptor with Pseudomonas exotoxin, J. Cel. Phys. 120:271-279 (1984).
Bacha et al., Thyrotropin-releasing hormone-diptheria toxin-related polypeptide conjugates, J. Biol. Chem. 258:1565-1570 (1983).
Bacha et al., Organ-specific binding of a thyrotropin-releasing hormone-diptheria toxin complex after intravenous administration to rats, Endocrinology 113:1072-1076 (1983).
Baird et al., Molecular characterization of fibroblast growth factor: Distribution and biological activities in various tissues, Recent Progress in Hormone Res. 42:143-205 (1986).
Baird et al., Receptor-and heparin-binding domains of basic fibroblast growth factor, P.N.A.S. 85:2324-2328 (1988).
Bergamaschi et al., Killing of K562 cells with conjugates between human transferrin and a ribosome-inactivating protein (SO-6), British J. of Haematology 68:379-384 (1988).
Biro et al., "Stimulation and inhibition of protein synthesis in endothelial and smooth muscle cells by bFGF-saporin," New York Acad. Sci. Abstracts, p. 16, Jan. 16-18, 1991.
Bregni et al., Activity of a Monoclonal antibody-saporin-6 conjugate against B-lymphoma cells, J. National Cancer Institute, 80:511-517 (1988).
Case et al., Chimeric cytotoxin IL2-Pe40 delays and mitigates adjuvant-induced arthritis in rats, P.N.A.S., 86:287-291 (1989).
Chaudhary et al., Activity of a recombinant fusion protein between transforming growth factor type alpha and Pseudomonas toxin, P.N.A.S., 84:4538-4542 (1987).
Chaudhary et al., Selective killing of HIV-infected cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein, Nature, 355:369-372 (1988).
Cuevas et al., Basic fibroblast growth factor (FGF) promotes cartilage repair in vivo, Biochem. and Biophys. Res. Comm., 156:611-618 (1988).
Esch et al., Primary structure of bovine brain acidic fibroblast growth factor (FGF), Biochem. and Biophys. Res. Comm., 133;554-562 (1985).
Esch et al., Primary structure of bovine pituitay basic fibroblasts growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF, P.N.A.S. 82:6507-6511 (1985).
FitzGerald et al., Adenovirus-induced release of epidermal growth factor and Pseudomonas into the cytosol of KB cells during receptor-mediated endocytosis, Cell, 32;607-617 (1983).
Folkman et al., Angiogenic factors, Science, 235;442-447 (1987).
Grindey, "Current status of cancer drug development: Failure of limited success?," Cancer Cells 2(6):163-171 (1990).
Halaban et al., bFGF as an autocrine growth factor for human melanomas, Oncogene Research, 3:177-186 (1988).
Kelley et al., Interleukin 2-diphtheria toxin fusion protein can abolish cell-mediated immunity in vivo, P.N.A.S., 85:3980-3984 (1988).
Lappi et al., Biological and chemical characterization of basic EGF-saporin mitotoxin, Biochem. Biophys. Res. Commun., 160:917-923 (1989).
Lorberboum-Galski et al., Cardiac allograft survival in mice treated with IL-2-PE40, P.N.A.S., 86:1008-1012 (1989).
Lorberboum-Galski et al., Cytotoxic activity of an interleukin 2-Pseudomonal exotoxin cimeric protein produced in Escherichia coli, P.N.A.S., 85:1922-1926 (Mar. 1988).
Lorberboum-Galski et al., Interleukin 2 (IL2) PE40 is cytotoxic to cells displaying either the p55 or p70 subunit of the IL2 receptor, J. Biol. Chem. 263:18650-8656 (1988).
Schwartz et al., A new cytotoxin specific for the target cells of corticotropin-releasing factor, Endocrinology 121:1454-1460 (1987).
Siegall et al., FASEB Journal, 5:2843-2849 (1991).
Siegall et al., Cytotoxic activities of a fusion comprised of TGFalpha and Pseudomonas exotoxin, FASEB Journal 3:2647-2652 (1989).
Siegall et al., Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin, P.N.A.S., 85:9738-9742 (1988).
Siena et al., Evaluation of antihuman T lymphocyte saporin immunotoxins potentially useful in human transplantation, Transplantation, 46:747-753 (1988).
Siena et al., Synthesis and characterization of an antihuman T-lymphocyte saporin immunotoxin (OKT-1-SAP) with in vivo stability into nonhuman primates, Blood, 72:756-765 (1988).
Taetle et al., Effects of anti-epidermal growth factor (EGF) receptor antibodies and an anti-EGF receptor recombinant-ricin A chain immunoconjugate on growth of human cells, J. Nat. Cancer Inst., 80(13):1053-1059 (1988).
Walicke, et al., Neurotrophic effects of basic and acidic fibroblast growth factors are not mediated through glial cells, Developmental Brain Research, 40:71-79 (1988).
Vollmar et al., Toxicity of ligand and antibody-directed ricin A-chain conjugates recognizing the epidermal growth factor receptor, J. Cell. Phys. 131:418-425 (1987).
Baird J. Andrew
Lappi Douglas A.
The Salk Institute for Biological Studies
Walsh Stephen G.
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