Fibrin binding domain polypeptides and uses and methods of produ

Details Fibrin binding domain polypeptides and uses and methods of produ Fibrin binding domain polypeptides and uses and methods of produ

1993-05-04

1995-10-03

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

424 169, 424 9341, 424 94, 435 78, 435 13, 436503, 436504, 436 69, G01N 3353, A61K 5108, C12Q 156

Patent

active

054551587

ABSTRACT:
This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imaging a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and which are capable of binding to fibrin.

REFERENCES:
patent: 4315906 (1982-02-01), Gelder
patent: 5026537 (1991-06-01), Daddiba et al.
Uehara et al, 1988, Iodine-131-Labeled Fibronectin: Potential Agent for Imaging Atherosclerotic Lesion and Thrombus. J. Nucl. Med. 29:1264-7.
Garcia-Pardo et al, 1983, Primary Structure of Human Plasma Fibronectin, The 29,000 Dalton NH.sub.2 -Terminal Domain, J. Biol. Chem 258: 12670-4.
Mosher et al, 1980. Binding and Factor XIII.sub.a -Mediated Cross-Linking of a 27-Kilodalton Fragment of Fibronectin to Staphylococcus aureus. Sci 209:927-9.

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