Fibrin binding domain polypeptide and method of producing

Details Fibrin binding domain polypeptide and method of producing Fibrin binding domain polypeptide and method of producing

1990-05-21

1993-12-14

Ossanna, Nina

Drug, bio-affecting and body treating compositions

In vivo diagnosis or in vivo testing

Magnetic imaging agent

530350, 530395, 4353201, 435 691, 4352523, 43525233, 4352401, 424 11, A61K 9900, C07K 1300, C12N 1574, C12P 2102

Patent

active

052700305

ABSTRACT:
This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imaging a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and which are capable of binding to fibrin.

REFERENCES:
patent: 4315906 (1982-02-01), Gelder
patent: 4455290 (1984-06-01), Olexa et al.
patent: 4587122 (1986-05-01), Kagitani et al.
patent: 4663146 (1987-05-01), Morser et al.
patent: 4839464 (1989-06-01), McCarthy et al.
Uehara et al., J. of Nuclear Medicine, 29:1264-7, (Jul. 1988).
Hynes and Yamada, J. of Cell Biology, 95:369-377, (Nov. 1982).
Garcia-Pardo et al., J. Biol. Chem., 258:12670-12674, (1983).
Mosher and Procter, Science, 209:927-929, (Aug. 1980).

Affiliated with

Also associated with

Rating

Fibrin binding domain polypeptide and method of producing does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fibrin binding domain polypeptide and method of producing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fibrin binding domain polypeptide and method of producing will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1703233