Fiber optic apparatus for detecting light scatter to...

Optics: measuring and testing – By particle light scattering – With photocell detection

Reexamination Certificate

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C356S340000, C356S342000, C422S082050, C422S082090, C436S164000

Reexamination Certificate

active

06922241

ABSTRACT:
Apparatus for detecting light scattered by a small particle (e.g., a blood cell) irradiated by a light beam comprises one or more photodetectors and a plurality of optical fibers that serve to optically couple the scattered light and the photodetector(s). To enhance the efficiency of such optical coupling, a portion of each of the optical fibers in the vicinity of its light-collecting end is supported so that its optical axis extends towards the light-scattering source. By this arrangement, scattered light enters each fiber from a direction substantially parallel to the fiber axis. Preferably, the light-collecting ends of the optical fibers are supported on a concave surface and so that the respective optical axes of the fibers converge at a point representing the apparent position of the light-scattering source, taking into account the refractive effects of an optical flow cell through which scattering is detected. Preferably, both forward- and back-scattered light are detected by the apparatus of the invention.

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Eisert, W.G., “Cell Differentiation Based on Absorption and Scattering”, The Journal of Histochemistry and Cytochemistry, vol. 27, No. 1, pp. 404-409 (1979).

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