Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-12-12
2010-12-28
Allen, Marianne P (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C536S023500, C435S320100, C435S252800, C530S412000, C530S416000, C530S417000
Reexamination Certificate
active
07858341
ABSTRACT:
The expression vectors and methods using anE. coliexpression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation inE. coli. Using the expression vectors, the FGF18 gene was produced inE. colito a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientE. colistrains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.
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Chan Chung-leung
Julien Susan H.
Liu Hong Y.
Reardon Brian J.
Allen Marianne P
Woodcock & Washburn LLP
ZymoGenetics Inc.
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