Fetal lung maturity test employing lamellar body solubilization

Details Fetal lung maturity test employing lamellar body solubilization Fetal lung maturity test employing lamellar body solubilization

1986-05-30

1989-04-11

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

436 2, 436 34, 436 71, 436164, 436172, C12Q 100

Patent

active

048206288

ABSTRACT:
The lamellar body solubilization test (LBST) is a rapid and simple indicator of fetal lung maturity. The amniotic fluid contains lamellar bodies, the presence of which can be correlated to maturation of lung tissue. The test is conducted by measuring a difference in an optical property (turbidity, light scatter, innate or added fluorescence) of a sample of amniotic fluid or other physical property affected by the presence of particulate bodies suspended in a liquid, either 1) kinetically (rate method) or 2) as an end point method (pre- and post-), due to the unravelling or solubilization of the lamellar bodies caused by subjecting the sample to a physical or chemical agent, e.g., detergent or heat. The change in that property either rate of change or delta, by the destruction of the lamellar body structure provides an accurate indication of the concentration and size of the lamellar bodies in the sample of amniotic fluid, which in turn provides a good indication of fetal lung maturity.

REFERENCES:
Oulton, "The Role of Centrifugation . . . in Amniotic Fluid", Am. J. Obstet. Gynecol., Oct. 1, 1979, pp. 337-343.
Oulton et al., "Assessment of Fetal Pulmonary Maturity . . . Lamellar Bodies", Am. J. Obstet. Gynecol. 03/15/82, pp. 684-691.
Oulton et al., "Developmental Study . . . From Human Amniotic Fluid", Pediatr. Res. 14: 722-728 (1980).
CRC Critical Reviews in Clinical Laboratory Sciences, vol. 16, Issue 2, Jan. 1982, pp. 85-157.
Tsai et al., "Absorbance of Amniotic Fluid . . . Tests for Disaturated Phosphatidylcholine and Phosphatidylglycerol", Am. J. Obstet. Gynecol., Aug. 15, 1983, pp. 963-966.
Weitz, et al., Clin. Chem., vol. 31, No. 6, (1985), p. 951.

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