Fetal cell recovery method

Details Fetal cell recovery method Fetal cell recovery method

1990-03-27

1992-10-06

Saunders, David

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 6, 435 721, 435 724, 435 29, 435 30, 43524021, 435243, 436503, 436800, 436824, C12N 100, C12Q 124

Patent

active

051531178

ABSTRACT:
The present invention provides a method for selectively recovering fetal cells from a maternal blood sample. The method comprises the following steps. Cells of the sample are combined with a first and a second antibody labeled with different fluorochromes for a period of time sufficient for antibody binding to produce labeled cells. The antibodies are specific for two different antigens expressed by two material HLA alleles. In a preferred embodiment, the alleles are of an HLA locus for which the woman is heterozygous. Cells having two different fluorescent labels are separated from cells having either a single fluorescent label or unlabeled cells using fluorescence-activated cell sorting. The separated single-labeled and unlabeled cells are recovered. The separated fetal cells can be used in a variety of procedures including DNA amplification methods and karyotyping.

REFERENCES:
patent: 4675286 (1987-06-01), Calenoff
patent: 4835098 (1989-05-01), Orr et al.
patent: 4987086 (1991-01-01), Brosnan et al.
I. M. Roitt, Essential Immunology, 5th Edition, Blackwell Scientific Publications, Oxford UK, 1984, pp. 278-279.
L. A. Herzenberg et al, Proc. Natl. Acad. SCI U.S.A., 76, 1453-1455, 1979.
Iverson et al, Prenatal Diagnosis, 61:61-73 (1981).
Bianchi, et al, Cytometry, 8:197-202 (1987).

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