Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1985-03-01
1987-07-14
Jones, Larry
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435291, 435315, 435807, C12M 136
Patent
active
046802673
ABSTRACT:
A method and an apparatus for controlling the concentration of oxygen and pH of a medium during a bioreaction process in a vessel. The method and apparatus are particularly adapted to a fermentation process. A dissolved oxygen sensor generates a signal corresponding to the pH of the medium and a dissolved oxygen sensor generates a signal corresponding to the dissolved oxygen in the medium. A valve mechanism selectively applies air, N.sub.2, O.sub.2 and CO.sub.2 to the medium. A controller produces a control signal for controlling the operation of the valve means so that a substantially fixed volume of gas consisting of one or more of air, CO.sub.2, N.sub.2 and O.sub.2 are added to the fermenting medium during a period of time. The controller determines in response to the dissolved oxygen and pH signals, the amount of CO.sub.2, O.sub.2 and/or N.sub.2 required to effect dissolved oxygen and pH correction. The controller further adjusts these determined values to compensate for the displacement of the CO.sub.2 added. As a result, the effect of the CO.sub.2 correction on the dissolved oxygen is substantially minimized.
REFERENCES:
patent: 3926738 (1975-12-01), Nyiri et al.
patent: 3941662 (1976-03-01), Munder et al.
patent: 4154652 (1979-05-01), Sawamura et al.
patent: 4424559 (1984-01-01), Lorincz et al.
Eppstein Lee B.
Mohler Robert D.
Reuveny Shaul
Jones Larry
Kamen Noah
New Brunswick Scientific Company, Inc.
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