Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus
Patent
1999-04-23
2000-09-26
Nashed, Nashaat
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing a carotene nucleus
435183, 4352523, 43525233, 435410, 4353201, 536 232, 536 237, C12P 2300
Patent
active
06124113&
ABSTRACT:
Novel proteins of Flavobacrerium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially .beta.-carotene, lycopene, zeaxanthin and cantaxanthin. Processes are also provided for preparing zeaxanthin by culturing a transformed host cell containing an expression cassette that includes a polynucleotide having a DNA sequence which encodes the GGPP synthase of Flavobacterium sp. R1534 (crtE), the prephytoene synthase of Flavobacterium sp. R1534 (crtB), the phytoene desaturase of Flavobacterium sp. R1534 (crtI), the lycopene cyclase of Flavobacterium sp. R1534 (crtY), or the .beta.-carotene hydroxylase of Flavobacterium sp. R1534 (crtZ). The polynucleotide is substantially free of other polynucleotides of Flavobacterium sp. R1534. The process further includes isolating the zeaxanthin from such cells or the culture medium.
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Derwent English language abstract of CH 595 444 A.
Hohmann Hans-Peter
Pasamontes Luis
Tessier Michel
van Loon Adolphus
Haracz Stephen M.
Nashed Nashaat
Roche Vitamins Inc.
Waddell Mark E.
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