Fermentation process for the production of pyrimidine deoxyribon

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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4351723, 4352523, 43525233, 4353201, 536 232, 935 14, 935 60, 935 68, 935 72, 935 73, C12N 1500, C12N 1570, C12N 1555, C12P 1930

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052139727

ABSTRACT:
DNA coding for at least one enzyme that causes the accumulation of a pyrimidine deoxyribonucleoside is used, in conjunction with metabolic mutations or heterologous DNA coding for metabolic enzymes that also increase pyrimidine deoxyribonucleoside production, to engineer cultured cells to express a pyrimidine deoxyribonucleoside (PdN) in recoverable quantities, providing a commercially useful fermentation source for PdNs.

REFERENCES:
Fukui & Tanaka, Advances in Biochemical Engineering, 17: 1-35 (1980).
Wang et al. "Uracil-DNA Gylcosylase Inhibitor of Bacteriophage PBS2: Cloning and Effects of Expression of Inhibitor Gene in Escherichia coli", J. Bacteriology, 1082-1091 (1988).
Aposhian, "A dTMPase Found After Infection of Bacillus subtilis with Phage SP5C," Biochem. and Biophys. Res. Comm., vol. 18, No. 2 (1965) pp. 230-235.
Aposhian & Tremblay, "Purification and Properties of an Enzyme Found After Infection of Bacillus subtilis with Phage SP5C," Journal of Biological Chemistry, vol. 241, No. 21 (1966), pp. 5096-5101.
Beckwith et al., "Coordination of the Synthesis of the Enzymes in the Pyrimidine Pathway of E. coli," J. Mole. Biol. (1962), pp. 618-634.
Bethell & Jones, "Molecular Size and Feedback-Regulation Characteristics of Bacterial Aspartate Transcarbamylases," Archives of Biochem. and Biophys., vol. 134 (1969), pp. 352-365.
Haslam et al., "Inhibition of Thymidylate Synthetase in Bacteriophage Infected Bacillus subtilis," Biochim, Biophys. Acta., vol. 134 (1967) pp. 312-326.
Hayward, "Inhibition of Bacerial DNA and Protein Synthesis in Bacillus subtilis by Phage SP82. Effect of Changes of Temperature on the Inhibition," Virology, vol. 38 (1969), pp. 538-549.
Hemphill & Whiteley, "Bacteriophages of Bacillus subtilis," Bacteriological Review, vol. 39 (1975), pp. 357-315.
Kunkel et al., "Rapid and Efficient Site-Specific Mutagenesis Without Phenotypic Selection," Methods in Enzymology, vol. 154 (1987), pp. 367-382.
Landick & Yanofsky, "Transcription Attenuation," E. Coli and Salmonella Typhimurium Cellular & Molecular Biology, vol. 2, pp. 1276-1301.
Lerner & Switzer, "Cloning and Structure of the Bacillus subtilis Aspartate Transcarbamylase Gene (pyrB)," Journal of Biological Chemistry, vol. 261, No. 24 (1986), pp. 11156-11165.
Lerner et al., "Structure of the Bacillus subtilis Pyrimidine Biosynthetic (pyr) Gene Cluster," Journal of Bacteriology (May, 1987), pp. 2202-2206.
Munch-Petersen, "Nucleoside Catabolism," Metabolism of Nucleotides, Nucleosides and Nucleobases in Microorganisms (1983), pp. 203-258.
Neuhard, "Regulation of Pyrimidine Nucleotide Biosynthesis in Escherichia coli and Salmonella Typhimurium," The Molecular Biology of Bacterial Growth, pp. 173-184.
Neuhard & Nygaard, "Purines and Pyrimidines," Biosynthesis and Conversions of Nucleotides (1987), pp. 445-473.
Nishihara et al., "The Deoxycytidylate Deaminase Found in Bacillus subtilis Infected with Phage SP8," Biochem., vol. 6, No. 7 (1967), pp. 1877-1886.
O'Donovan & Neuhard, "Pyrimidine Metabolism in Microorganisms," Bacteriological Reviews, vol. 34, No. 3, (1970), pp. 278-343.
Paulus & Switzer, "Characterization of Pyrimidine-Repressible and Arginine-Repressible Carbamyl Phosphate Synthetases from Bacillus subtilis," Journal of Bacteriology, vol. 137, No. 1 (1979), pp. 32-91.
Potvin, et al., "Pyrimidine Biosynthetic Pathway of Bacillus subtilis," Journal of Bacteriology, vol. 123 (1975), pp. 604-615.
Roland, et al., "Role of Translation and Attenuation in the Control of pyrBI Operon Expression in Escherichia coli K-12," Journal of Bacteriology, vol. 163, No. 3 (1985), pp. 991-999.
Roscoe & Tucker, "The Biosynthesis of a Pyrimidine Replacing Thymine in Bacteriophage DNA," Biochem., and Biophys. Res. Comm., vol. 16, No. 2 (1964), pp. 106-110.
Roscoe & Tucker, "The Biosynthesis of 5-Hydroxymethyldeoxyuridylic Acid in Bacteriophage-Infected Bacillus subtilis," Virology, vol. 29 (1966), pp. 157-166.
Rowlands, "Industrial Strain Improvement: Mutagenesis and Random Screening Procedures," Enzyme Microb. Technol., vol. 6 (1984), pp. 3-10.
Shepherdson & Pardee, "Production and Crystallization of Aspartate Transcarbamylase," Journal of Biol. Chem., vol. 235, No. 11 (1960), pp. 3233-3237.
Takahashi & Marmur, "Replacement of Thymidylic Acid by Deoxyuridylic Acid in the Deoxyribonucleic Acid of a Transducing Phage for Bacillus subtilis," Nature, vol. 197 (1963), pp. 794-795.
Thelander & Reichard, "Reduction of Ribonucleotides," Ann. Rev. Biochem., vol. 48 (1979), pp. 133-158.
Turnbough et al., "Attenuation Control of pyrBI Operon Expression in Escherichia coli K-12," Proc. Natl. Acad. Sci. USA, vol. 80 (1983), pp. 368-372.
Yakobsen & Guiney, "Conjugal Transfer of Bacterial Chromosomes Mediated by the RK2 Plasmid Transfer Origin Cloned into Transporon Tn5," Journal of Bacteriology, vol. 160, No. 1 (1984), pp. 451-453.
Nowlan, S. F., et al., 1985, The Journal of Biological Chemistry, 260(27):14712-14716.
Levin, H. L., et al., 1985, Proceedings of the National Academy of Sciences USA 82:4643-4647.
Fauve, M., et al., 1989, European Journal of Biochemistry 179:345-358.
Nowlan, S. F., et al., 1983, Molecular and General Genetics, 192:264-271.
Jensen, K. F., 1988, European Journal of Biochemistry, 175:587-593.
Turnbrough, Jr., C. L., 1983, Journal of Bacteriology, 153:998-1007.
Navre, M., et al., 1983, Proceedings of the National Academy of Sciences USA, 80:1207-1211.
Jensen, K. F., et al., 1982, The EMBO Journal 1(1):69-74.
Miyagawa, K., et al., 1989, Bio/Technology 7:821-824.
Miyagawa, K., et al., 1986, Bio/Technology 4:225-228.
Matsui, H., et al., 1977, Applied and Environmental Microbiology 34(4):337-341.

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