Fermentation process for the production of polysaccharides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435101, 435 3, 435813, 435819, 435910, 435244, C12P 1908, C12P 1904, C12Q 300, C12N 138, C12R 164

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046924086

ABSTRACT:
Polysaccharides, such as xanthan gum, are produced by culturing microorganisms, e.g. of the Xanthomonas genus, in a two stage process. In the first stage, growth of the microorganism is favored, e.g. by using a predetermined quantity of a carbon-containing nutrient which does not support biosynthesis of the polysaccharide. In the second stage, the conditions are such that biosynthesis of the polysaccharide takes place with substantially no growth of the microorganism, e.g. by adding carbohydrate in the absence of nutrient required for polysaccharide growth.
By this process, the requirement for oxygen is greatly reduced at the time when the culture medium has its highest viscosity, thereby minimizing problems of low oxygen transfer capability in viscous media.

REFERENCES:
patent: 3251749 (1966-05-01), Lipps, Jr.
patent: 4355106 (1982-10-01), Lawford
patent: 4400466 (1983-08-01), Azoulay
patent: 4400467 (1983-08-01), Bauer et al.
G. W. Pace and R. C. Righelato, "Advances in Biochemical _Engineering" vol. 15, 1980, pp. 29-68.

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