Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-05-27
2010-02-23
Ramirez, Delia M (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S116000, C435S252330, C435S069100, C435S320100, C530S350000, C536S023100
Reexamination Certificate
active
07666634
ABSTRACT:
The present invention relates to a fermentation process for the preparation of L-amino acids in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms' open reading frames of yjfA and ytfP are individually or jointly inactivated by one or more methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or tranversion mutagenesis with incorporation of a non-sense mutation in the yjfA and ytfP gene region; (b) concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acids; and (c) isolation of the L-amino acids.
REFERENCES:
patent: 1094111 (2000-10-01), None
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Bastuck Christine
Hermann Thomas
Rieping Mechthild
Thierbach Georg
Evonik Degussa GmbH
Pillsbury Winthrop Shaw & Pittman LLP
Ramirez Delia M
LandOfFree
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