Fermentation process for producing higher plant cells

Details Fermentation process for producing higher plant cells Fermentation process for producing higher plant cells

1979-08-08

1982-04-20

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435818, C12N 502

Patent

active

043260349

ABSTRACT:
Higher plant cells (e.g. cells of Spermatophyta) capable of growing in the presence of light and in the absence of a carbohydrate carbon source are produced from higher plant cells which require a carbohydrate source by cultivation in an aqueous medium in the presence of light, O.sub.2 and CO.sub.2. The quantity of carbohydrate is reduced during the cultivation while the concentration of dissolved oxygen is maintained at a value below 250 n mol of O.sub.2 per ml of medium.
Preferably cultivation is in continuous culture and the preferred dissolved O.sub.2 concentration is from 25 to 85 n mol per ml. The CO.sub.2 present and the quantum flux density may be increased as the carbohydrate is reduced.

REFERENCES:
patent: 3816960 (1974-06-01), Gudin et al.
American Journal of Botany, vol. 50, pp. 248-254 (1963).

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