Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...
Reexamination Certificate
2005-01-18
2005-01-18
Lilling, Herbert J. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing heterocyclic carbon compound having only o, n, s,...
C435S123000
Reexamination Certificate
active
06844174
ABSTRACT:
The present invention provides a fermentation process for producing lipstatin comprising the steps of: a) preparing a fermentation medium containing a lipstatin-producing microorganism comprising an oil and an assimilable carbon source, wherein the wt/wt ratio of oil and assimilable carbon source is regulated to achieve an optimal lipstatin biosynthesis by the microorganism; and b) feeding the fermentation medium with an emulsifier, wherein the emulsifier provides an optimal viscosity for the fermentation medium and optimal pH during the fermentation to permit fermentation for lipstatin production. The disclosed process also provides a process for extracting a lipstatin from a fermentation broth.
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E.K. Weibel, et al. “Lipstatin, an inhibitor of pancreative lipase, produced by Streptomyces Toxytricini. Part I” the Journal of Antibiotics, vol. XL, No. 8, p. 1081-1085, (1987).
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Balogh Gabor
Csorvasi Andrea
Erdei Janos
Gulyas Eva
Keri Vilmos
Biogal Gyogyszergyar Rt.
Kenyon & Kenyon
Lilling Herbert J.
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