Fermentation process for creatinine iminohydrolase

Details Fermentation process for creatinine iminohydrolase Fermentation process for creatinine iminohydrolase

1989-08-14

1991-09-03

Lee, Lester L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352521, 4352531, 435850, C12N 978

Patent

active

050454650

ABSTRACT:
A fermentation process and improved aqueous nutrient medium are used for the production of urease-free creatinine iminohydrolase from an aerobic soil microorganism. In the process, an inoculum culture of the microorganism is transferred into a production medium to generate microorganisms in which creatinine iminohydrolase production has been induced, said nutrient medium containing a source of ammonia and after the ammonia is substantially exhausted, incrementally adding a solution of creatinine, controlling the pH by addition of .alpha.-ketoglutaric acid, and extracting urease-free creatinine iminohydrolase. An improved aqueous nutrient medium for use as the production medium is disclosed.

REFERENCES:
patent: 4087329 (1978-05-01), Terada et al.
patent: 4134793 (1979-01-01), Terada et al.
patent: 4275164 (1981-06-01), Masurekar
patent: 4276377 (1981-06-01), Goodhue et al.
Crueger, W. and Crueger, A., 1982, Biotechnology: A Textbook of Industrial Microbiology, Brock, D. (ed.) Science Tech, Inc.
J. Szulmajster, J. Bacteriology, "Bacterial Fermentation of Creatinine", vol. 75, pp. 633-639, (1958).
J. Szulmajster, Biochimica et Biophysica Acta, "Bacterial Degradation of Creatinine", vol. 30, pp. 154-163, (1958).

Affiliated with

Also associated with

Rating

Fermentation process for creatinine iminohydrolase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fermentation process for creatinine iminohydrolase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fermentation process for creatinine iminohydrolase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1008872