Fermentation of pentose sugars

Details Fermentation of pentose sugars Fermentation of pentose sugars

2009-10-15

2011-11-15

Fronda, Christian (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S183000, C435S233000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

08058040

ABSTRACT:
The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

REFERENCES:
patent: 5643758 (1997-07-01), Guan et al.
patent: 5858764 (1999-01-01), De Hollander
patent: 5935837 (1999-08-01), Rasmussen
patent: WO 9624667 (1996-08-01), None
patent: WO 00/61733 (2000-10-01), None
patent: WO2004/099381 (2004-11-01), None
patent: WO2006/009434 (2006-01-01), None
patent: WO2010/074577 (2010-06-01), None
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Database EMBL ‘Online!’; Mar. 3, 2000; Op Den Camp, HJM; “Piromycessp. E2 mRNA for xylose isomerase (xylA gene)”; Database accession No. AJ249909.
Database EMBL ‘Online!’ Mar. 3, 2000; Op Den Camp HJM;Piromycessp. E2 mRNA for D-xylulokinasc (xylB gene); Databasc accession No. AJ249910.
Zaldivar, J et al., “Fucl cthanol production from lignoccllulosc: A challcngc for mctabolic engineering and process integration.”;Appl Microbiol Biotechnol.56:17-34 (2001).
Teunissen. Mj et al., “Production of cellulolytic and xylanolytic enzymes during growth of the anaerobic fungusPiromyces• Sp on different substrates”;J. Gen. Microbiol. 138:657-1664 (1992).
Chica et al. Curr Opin Biotechnol ( Aug. 2005) 16:378-84.
Witkowski et al. . . .Biochemistry(Sep. 1999) 38:11643-50.
Sefternick et al.J Bacteriol. (Apr. 2001) 183:2405-10.
Accession Q9P8C9, published Oct. 1, 2000.
Karlsson et al.Eur J Biochem. (Dec. 2001) 268:6498-6507.
Bhosale, S.H., Rao, M.B., Deshpande, V.V: Molecular and Industrial Aspects of Glucose Isomerase,Microbiol Rev. 60:280-300 (1996).
Meaden, PG, et al., The Xylose Isomerase—Encoding Gene (XylA) ofClostridium thermosaccharolyticum: Cloning, Sequencing . . . of Xy1A Enzymes,Gene 141:97-101 (1994).
Herrick, K., Blow, D.M., Carrel H.L.I., Glusker, J.P.: Comparison of Backbone Structures of Glucose Isomerase fromStreptomycesandArthobacter. Protein Engineering 1:467-469 (1987).
Henrick K et al.: Structures of o-xylose Isomerase fromArthrobacterStrain B3728 Containing the Inhibitors Xylitol and D-Sorbitol . . .J Mol. Biol. 208:129-157 (1989).
Vangrysperre, W., Van Damrne J., Vandekerckhove J., et al., Localization of the Essential Histidine and Carboxylate Group in Xylose Isomerases.Biochem J. 265:699-705 (1990).
Bruinenberg P.M., de Bot, P., van Dijken, J., Scheffers, WA.: The Role of Redox Balances on the Anaerobic Fermentation of Xylose by Yeasts.Eur. J Appl. Microbiol. Biotechnol. 18:287-292 (1983).
Gardonyi, M. and Hahn-Hagerdal, B.: TheStreptomyces rubiginosusXylose Isomerase is Misfolded when Expressed inSaccharomyces cerevisiae. Enz. Microb. Technol. 32:252-259 (2003).
Amore R. et al., The Fermentation of Xylose—an Analysis of the Expression ofBacillusandActinoplanesXylosc Isomcrasc Genes in Yeast,Appl Microbiol. Biotechnol. 30:351-357 (1989).
Chan, E-C., Ueng, P.P., Chen, L.F.: Metabolism of D-Xylose inSchizosaccharomycesPombe cloned with a Xylose Isomerase Gene,Appl. Bicrobiol. Biotechnol. 31: 524-528 (1989).
UNIPROT Database listing of available xylose isomerase sequences as of Jan. 22, 2002. (D25 of EPO Oppos).
Ucng et al., “Molccular cloning of theEscherichia coligcnc cncoding xylosc isomcrasc”,Biotech. Lett. 7:153-158 (1985). (D15 in EPO Oppos).
Chan, E-C. et al., “D-xylose fermentation to ethanol bySchizosaccharomyces pombecloned with xylose isomerase gene”,Biotechnol. Lett.8:231-234 (1986). (D13 in EPO Oppos).
Walfridsson et al.., “Ethanolic fermentation of xylose withSaccharomyces cerevisiaeharboring theThermus thermophilus xylAgene, which expresses an active xylose (glucose) isomerase”,Appl. Environ. Microbiol.62:4648-4651 (1996). (D8 in EPO Oppos).
Kristo et al.Eur. J. Biochem. 237:240-246 (1996) (D23 in EPO Oppos).
Harhangi et al., “Xylosc mctabolism in the anacrobic fungusPiromycessp. strain E2 follows thc bacterial pathway”.Arch. Microbiol. 180:134-141 (Jun. 13, 2003) (D7 in EPO Oppos).
Madhavan, A. el al., Xylose isomerase from polycentric fungusOrpinomyces: gene sequencing, cloning, and expression inSaccharomyces cerevisiaefor bioconversion of xylose to ethanol.Appl Microbiol Biotechnol. 82:1067-78 (Apr. 2009; Epub Dec. 3, 2008).

Affiliated with

Also associated with

Rating

Fermentation of pentose sugars does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fermentation of pentose sugars, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fermentation of pentose sugars will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4274801