Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Patent
1993-04-01
1993-11-09
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
435 691, 435 696, 435812, C12P 2100, C12N 1514
Patent
active
052602028
ABSTRACT:
The recoverable yield of recombinant human albumin in the fermentation of genetically engineered yeast that expresses the albumin and secretes it into the culture medium is increased by the addition of a stabilizing agent comprising a polyoxyalkylene compound, for example, polypropylene glycol or a polyoxypropylene/polyoxyethylene copolymer. The presence of these compounds in concentrations of from about 0.5 to 10 g per liter, preferably 1 to 5 g per liter, of fermentation broth prevents the degradation of the rHA produced under intensive fermentation conditions thereby raising the effective yield.
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Patent Cooperation Treaty, PCT/GB 89/01046, Sep. 6, 1989.
Clarke Peter M.
Collins Stephen H.
Mead David J.
Cassett Larry R.
Delta Biotechnology Limited
Lilling Herbert J.
Swope R. Hain
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