Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1989-06-28
1992-12-01
Marx, Irene
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
4352521, 435170, 562593, 562590, C12P 746, C12N 120, C07C 5142
Patent
active
051680556
ABSTRACT:
A process for economically producing highly purified succinic acid comprises growing a succinate-producing microorganism on a low cost carbohydrate substrate; simultaneously neutralizing the fermentation broth and precipitating the succinate as calcium succinate by adding a calcium ion source to form calcium succinate; isolating the calcium succinate; slurrying the calcium succinate in water and treating it with sulfuric acid to form calcium sulfate and succinic acid; and then treating the succinic acid with first a strongly acidic ion exchanger and then a weakly basic ion exchanger to remove impurities and obtain a highly purified succinic acid product. In a preferred embodiment, the calcium succinate is isolated from the fermentation broth by filtration; the filtrate is heated to precipitate additional calcium succinate; and, the spent filtrate which contains nutrients is recycled to the fermentor.
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Datta Rathin
Glassner David A.
Jain Mahendra K.
Vick Roy John R.
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