Feeding strategies for cell culture

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide

Reexamination Certificate

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C435S069100, C435S071100, C435S070300

Reexamination Certificate

active

06924124

ABSTRACT:
The invention is in the field of cell culture, particularly recombinant cell culture. More specifically, the invention relates to methods of fed batch CHO cell culture.

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Liu, Yung-Chuan, Cultivation of RecombinantE. colito Achieve High Cell Density with High Level of Pnencilin G Acylase Activity., Dec. 9, 1999, Proc. Natl. Sci Coun. vol. 24, No. 4. 2000. pp. 156-160.
deZengotita et al., “Phosphate Feeding Improves High-Cell-Concentration NS0 Myeloma Culture Performance for Monoclonal Antibody Production,”Biotechnology and Bioengineering 69(5):566-576, 2000.
Jo et al., “Step-Fortifications of Nutrients in Mammalian Cell Culture,”Biotechnology and Bioengineering 42:1218-1228, 1993.
Sato et al, “Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium,”Cytotechnology 2:63-67, 1989.

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