Feeding assay for identifying nematicidal compounds

Details Feeding assay for identifying nematicidal compounds Feeding assay for identifying nematicidal compounds

2006-04-18

2006-04-18

Witz, Jean C. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S243000, C424S009200

Reexamination Certificate

active

07029869

ABSTRACT:
The invention relates to any assay for the identification of nematicidal compounds such as anthelmintics for the control of nematodes. The assay comprises: (i) incubating a nematode sample (i.e. a “test nematode sample”) containing a pre-determined amount of nematodes with an ingestable substrate labelled with a readily detectable label, under conditions suitable for nematode feeding, wherein said incubation is conducted in the presence of said candidate nematicidal compound or follows a pre-incubation of said nematode sample with said candidate nematicidal compound, and (ii) determining whether said candidate nematicidal compound affects nematode feeding by detecting an increase or decrease in ingested substrate as compared to that which has been ingested by a control nematode sample which has been incubated without said candidate nematicidal compound.

REFERENCES:
patent: 5580749 (1996-12-01), Hughes
patent: 6006470 (1999-12-01), Geoghegan et al.
patent: 6540996 (2003-04-01), Zwaal et al.
patent: 6664441 (2003-12-01), Verwaerde et al.
patent: 6787125 (2004-09-01), Verwaerde et al.
De Coen et al. “The use of biomarkers inDaphinia magnatesting. III. Rapid toxicity testing of pure chemicals and sediment pore waters using ingestion and digestive enzyme activity” (1998) Chemosphere 37(13): 2677-2694.
Jones et al. “Feeding in inhibited by sublethal concentrations of toxicants and by heat stress in the nematodeCaenorhabditis elegans: Relations to the cellular stress response.” (Jul. 1, 1999) J. Exp. Zool. 284(2): 147-157.
Bockenhoff et al. Studies on the nutrient uptake by the beet cyst nematodeHeterodera schachiiby in situ microinjection of fluorescent probes into the feeding structures inArabidopsis thaliana. (1994) Parasitology 109: 249-254.
Boyd et al. “The effects of metals and food availability on the behavior ofCaenorhabditis elegans.” (2003) Environ. Toxicol. Chem. 22(12): 3049-3055.
Avery et al. “Effect of starvation and neuroactive drugs on feeding inCaenorhabditis elegans.” (1990) Exp. Zool. 253:263-270.
Hecht et al. “Simple adaptations to extend the range of flow cytometry five orders of magnitude fo the DNA analysis of uni- and multicellular systems.” (1981) 29(6): 771-774.
Hawdon et al. “Serum-stimulated feeding in vitro by third-stage infective larvae of the canine hookwormAncylostoma caninum.” (1990) J. Parasitol. 76(3): 394-398.
Moffat et al “Fluorescent lipid uptake and transport in adultSchistosoma mansoni.” (1992) 105:81-89.
Yeates, G.W. (1998) “Feeding in free-living soil nematodes: a functional approach.” In Perry, R.N., Wright D.J., eds, The Physiology and Biochemistry of Free-living and Plant parasitic nematodes. CAB International: New York, p. 245-269.
Haughland, R. Handbook of Fluorescent Probes and Research Chemicals 6th ed. (molecular Probes: Eugene, OR) 1996, p. 109-115.
Bansemir et al. (J. Parasitology (1994) 80(1): 24-28 (abstract only).
Webster's II New Reiverside University Dictionary (1994) (Houghton-Mifflin: Boston, MA) p. 1167.
Bansemir et al. “The Food Resource of AdultHeligmosomoides polygyrusin the Small Intestines” J. Parasitology (1994) 80(1): 24-28 (full paper).
Bottjer, K.P. and Bone, L.W. (1985), “Trichostrongylus Colubriformis: Effect of Anthelmintics on Ingestion and Oviposition,” Int. J. Paraasit. 15(5): 501-503.
Geary, T.G. et al. (1993), “Haemonochus contortus: Invermectin-inducted Paralysis of the Pharynx,” Exp. Parasit. 77:88-96.
Paiement, J-P et al. (Jul. 1999),Haemonchus contortus: Effects of Glutamate, Invermectin, and Moxidectin on Inulin Uptake Activity in Unselected and Invermectin-Selected Adults, Exp. Parasit. 92:193-198.
Brownlee, D.J.A. et al. (1997), “Actions of the anthelminitic invermectin on the pharyngeal muscle of the parasitic nematode,Ascaris suum,” Parasitology 115:553-561.
Bird, A.F. and Ryder, M.H. (1993), “Feeding of the NematodeAcrobeloides nanuson Bacteria,” J. Nematol. 25(3):493-499.

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