Feedback-resistant pyruvate carboxylase gene from...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S107000, C435S110000, C435S113000, C435S114000, C435S116000, C435S183000, C536S023200

Reexamination Certificate

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11136887

ABSTRACT:
The present invention relates to a mutated pyruvate carboxylase gene fromCorynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene inCorynebacteriumwith this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.

REFERENCES:
patent: 6171833 (2001-01-01), Sinskey et al.
patent: 6403351 (2002-06-01), Sinskey et al.
patent: WO/00/39305 (2000-07-01), None
PCT/US01/31893—ISR, Aug. 22, 2002, Hanke.
Attwood, P.V., “The Structure and the Mechanism of Action of Pyruvate Carboxylase,” Int. J. Biochem. Cell Biol. 27:231-249, Pergamon, Press(1995).
Modak, H.V., and Kelly, D.J. “Acetyl-CoA-dependent pyruvate carboxylase . . . chromatography.” Microbiology 141:2619-2628 (1995).
Peters-Wendisch et al. “Pyruvate carboxylase fromCorynebacterium glutamicum: characterization, expression and inactivation of the pyc gene,” Microbiology 144:915-927 (1998).
Koffas et al. (Dec. 24, 1997) GenBank accession AF038548.
Sinskey et al. (Oct. 20, 2000) Database Genseq. accession AABO1436, sequence alignment.

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