Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2007-11-27
2007-11-27
Slobodyansky, Elizabeth (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S107000, C435S110000, C435S113000, C435S114000, C435S116000, C435S183000, C536S023200
Reexamination Certificate
active
11136887
ABSTRACT:
The present invention relates to a mutated pyruvate carboxylase gene fromCorynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene inCorynebacteriumwith this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.
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patent: 6403351 (2002-06-01), Sinskey et al.
patent: WO/00/39305 (2000-07-01), None
PCT/US01/31893—ISR, Aug. 22, 2002, Hanke.
Attwood, P.V., “The Structure and the Mechanism of Action of Pyruvate Carboxylase,” Int. J. Biochem. Cell Biol. 27:231-249, Pergamon, Press(1995).
Modak, H.V., and Kelly, D.J. “Acetyl-CoA-dependent pyruvate carboxylase . . . chromatography.” Microbiology 141:2619-2628 (1995).
Peters-Wendisch et al. “Pyruvate carboxylase fromCorynebacterium glutamicum: characterization, expression and inactivation of the pyc gene,” Microbiology 144:915-927 (1998).
Koffas et al. (Dec. 24, 1997) GenBank accession AF038548.
Sinskey et al. (Oct. 20, 2000) Database Genseq. accession AABO1436, sequence alignment.
Guillouet Stephane
Hanke Paul D.
Sinskey Anthony J.
Willis Laura B.
Archer-Daniels-Midland Company
Buchanan & Ingersoll & Rooney PC
Cochenour, Esq. Craig G.
Massachusetts Institute of Technology
Slobodyansky Elizabeth
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