Feedback-resistant homoserine transsuccinylases

Details Feedback-resistant homoserine transsuccinylases Feedback-resistant homoserine transsuccinylases

2008-05-13

2008-05-13

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07371551

ABSTRACT:
The invention concerns a homoserine transsuccinylase having at least one mutation compared to a wild-type homoserine transsuccinylase and reduced sensitivity towards a L-methionine or SAM, compared to the wild-type enzyme. The latter comprises an amino acid sequence including a partial AspGlyXaaXaaXaaThrGlyAlaPro sequence between position 90 and position 115 and a partial TyrGlnXaaThrPro sequence between position 285 and position 310, position 1 of the amino acid sequence corresponding to the initial methionine. The invention is characterized in that the mutation is a substitution of the aspartate in the partial AspGlyXaaXaaXaaThrGlyAlaPro sequence, or a substitution of the tyrosine in the partial TyrGlnXaaThrPro sequence.

REFERENCES:
patent: 5120837 (1992-06-01), Fotheringham et al.
patent: 5698418 (1997-12-01), Brunner et al.
patent: 2002/0106800 (2002-08-01), Liaw et al.
patent: 0 745 671 (1996-12-01), None
patent: 2000-139471 (2000-05-01), None
Thomson Derwent corresponding to JP 2000-139471 A.
Morinaga et al., Agric. Biol. Chem., vol. 46, No. 1, 1982, pp. 57-63.
Bourhy et al., Journal of Bacteriology, vol. 179, No. 13, Jul. 1997, pp. 4396-4398.
Duclos, B. et al.,“Nucleotide sequence of the metA gene encoding homoserine trans-succinylase inEscherichia coli.” Nucleic Acids Research, vol. 17, No. 7, 1989, p. 2856.
Lawrence et al., Dept. of Genetics, vol. 58, Apr. 1968, pp. 473-492.
Kiedich et al., “The Enzymic Synthesis of L-Cysteine inEscherichia coliandSalmonella typhimurium,” J. Biol. Chem., 1966, vol. 241, No. 21, pp. 4955-4965.
Lee et al., “Multimetabolite Control of a Biosynthetic Pathway of Sequential Metabolities,” J. Biol. Chem., vol. 24, No. 22, 1966, pp. 5479-5480.
Shiozaki et al., Agric. Biol. Chem., vol. 53, No. 12. 1989, pp. 3269-3274.
Schlenk et al., “The Formation of S-Adenosylmethionine in yeast,” J. Biol. Chem., 1957, pp. 1037-1050.
Greene et al., “Biosynthesis of Methionine,” ASM Press, Washington 1996,Escherichia coliandSalmonella typhimurium, Second Edition, pp. 542-560.
Hornung et al., Proc. Natl. Acad. Sci. USA, vol. 96, Mar. 1999, pp. 4192-4197.
Born et al., Biochemistry, Oct. 1999, vol. 38, No. 43, pp. 14416-14423 as abstract.
Chattopadhyay et al. “Control of Methionine Biosynthesis inEscherichia coliK 12: a closer study with analoque-resistant mutants,” Journal of General Microbiology, 1991, vol. 137, pp. 685-691.
Chattopadhyay et al., Control of methionine biosynthesis inEscherichia coliK12: a closer study with analogue-resistant mutants, Journal of General Microbiology, 1991, pp. 685-691, vol. 137.

Affiliated with

Also associated with

Rating

Feedback-resistant homoserine transsuccinylases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Feedback-resistant homoserine transsuccinylases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Feedback-resistant homoserine transsuccinylases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2812202