Fatty acid desaturases and uses thereof

Details Fatty acid desaturases and uses thereof Fatty acid desaturases and uses thereof

2011-08-23

2011-08-23

McElwain, Elizabeth (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

C800S281000, C435S252300, C435S419000, C536S023200

Reexamination Certificate

active

08003853

ABSTRACT:
The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., SDA, EPA and DHA.

REFERENCES:
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patent: WO-03/099216 (2003-12-01), None
patent: WO 2005047479 (2005-05-01), None
patent: WO-2007/147256 (2007-12-01), None
Sequence alignment of SEQ ID No: 1 with Sequence Accession AEA14784, Damude et al, SEQ ID No: 3, run date May 18, 2010.
Damude, H. G., et al., “Identification of Bifunctional Δ12/ω3 Fatty Acid Desaturases for Improving the Ratio of ω3 to ω6 Fatty Acids in Microbes and Plants”, PNAS, 2006, vol. 103, No, 25, pp. 9446-9451.
“Subname: Full=Delta-12 Fatty Acid Desaturase”, EMBL Database, EBI Accession No. Q27ZJ7, Apr. 4, 2006.
Meesapyodsuk, D., et al., “Primary Structure, Regioselectivity, and Evolution of the Membrane-Bound Fatty Acid Desaturases ofClaviceps purpurea”, The Journal of Biological Chemistry, 2007, vol. 282, No. 28, pp. 20191-20199.
Meesapyodsuk, D., et al., “An Oleate Hydroxylase from the FungusClaviceps purpurea: Cloning, Functional Analysis, and Expression in Arabidopsis”. Plant Physiology, 2008, vol. 147, pp. 1325-1333.
International Search Report for Application No. PCT/CA2007/001218, dated Oct. 26, 2007.
Written Opinion for Application No. PCT/CA2007/001218, dated Oct. 26, 2007.

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