Fatty acid desaturases and mutant sequences thereof

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C800S264000, C800S270000

Reexamination Certificate

active

07541519

ABSTRACT:
Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

REFERENCES:
patent: 4627192 (1986-12-01), Fick
patent: 4948811 (1990-08-01), Spinner et al.
patent: 5387758 (1995-02-01), Wong et al.
patent: 5434283 (1995-07-01), Wong et al.
patent: 5545821 (1996-08-01), Wong et al.
patent: 5625130 (1997-04-01), Grant et al.
patent: 5629193 (1997-05-01), Hudson et al.
patent: 5859350 (1999-01-01), DeBonte et al.
patent: 5969169 (1999-10-01), Fan
patent: 6342658 (2002-01-01), DeBonte et al.
patent: 6372965 (2002-04-01), Lightner et al.
patent: 2180386 (1998-01-01), None
patent: 323 753 (1994-08-01), None
patent: WO 91/15578 (1991-10-01), None
patent: WO 92/03919 (1992-03-01), None
patent: WO 93/11245 (1993-06-01), None
patent: WO 94/11516 (1994-05-01), None
patent: WO 97/21340 (1997-06-01), None
GenBank Accession No. U50771 dated Apr. 4, 1996, 2 pages.
GenBank Accession No. X91139 dated Dec. 24, 2000, 2 pages.
“www.brassica.info sharing information world-wide for The Multinational Brassica Genome Project,” at http://www.brassica.info/information/GenomeSize.htm.
Arondel et al., “Map-Based Cloning of a Gene Controlling Omega-3 Fatty Acid Desaturation in Arabidopsis,”Science, 1992, 258:1153-1155.
Avelange-Macherel et al., “Site-directed mutagenesis of histidine residues in the Δ12 acyl-lipid desaturase of Synechocystis,”FEBS Letters, 1995, 361:111-114.
BRL Catalog and Reference Guide, “Sequencing Primers,” p. 61.
Brunklaus-Jung and Robbelen, “Genetical and Physiological Investigations on Mutants for Polyenoic Fatty Acids in Rapeseed (Brassica napus L.). III. Breeding Behaviour and Performance,”Plant Breeding, 1987, 98:9-16.
Canvin, “The Effect of Temperature on the Oil Content and Fatty Acid Composition of the Oils from Several Oil Seed Crops,”Can. J. Botany, 1965, 43:63-69.
Classen, Oral Presentation, GCIRC 8th International Rapeseed Congress, Saskatoon, Saskatchewan, Jul. 9-11, 1991, pp. 390-395.
Conner et al., “The Dietary Prevention and Treatment of Coronary Heart Disease,”Coronary Heart Disease: Prevention, Complications and Treatment, 1985, pp. 43-64.
Craig, “Varietal and Environmental Effects on Rapeseed. III. Fatty Acid Composition of 1958 Varietal Tests,”Can. J. Plant Sci., 1961, 41:204-210.
Downey et al., “Methods of Breeding for Oil Quality in Rape,”Can. J. Plant Sci., 1963, 43:271-275.
Doyle et al.,J. Biol. Chem., 1986, 261:9228-9238.
Evans et al., “Edible Oil Evaluation by Room Odor Tests: A Preliminary Report,”J. Am. Oil Chem. Soc., 1971, 48:711-714.
Fox et al., “Stearoyl-acyl carrier protein Δ9 desaturase from ricinus communis is a diiron-oxo protein,”Proc. Natl. Acad. Sci. USA, 1993, 90:2486-2490.
Galliard, “Degradation of Acyl Lipids: Hydrolytic and Oxidative Enzymes,”The Biochemistry of Plants, 1980, 4:85-116.
Garg et al.,New England J. Med., 1988, 319:829-834.
Gaul, “Mutations in Plant Breeding,”Radiation Botany, 1964, 4:155-232.
Grundy,New England J. Med., 1986, 314:745-748.
Hawrysh, “Stability of Canola Oil,”Canola and Rapeseed Production, Chemistry, Nutrition and Processing Technology, 1990, Chapter 7, pp. 99-122.
Hitz et al.,Plant Physiol., 1994, 103:635-641.
Hoffman et al., “Anther Culture as a Breeding Tool in Rape. II. Progeny Analyses of Androgenetic Lines and Induced Mutants from Haploid Cultures,”Theor. Appl. Genet., 1982, 61:225-232.
Jönsson et al., “Quality breeding in rapeseed,”Svalöv 1886-1986 Research and Results in Plant Breeding, Gösta Olsson (ed.), LTs forlag, Stockholm, pp. 173-184.
Keys (ed.),Circulation, vol. XLI, Suppl. I, 1970, pp. I162-I183.
Kirk-Othmer,Encyclopedia of Chemical Technol., 3rd Ed., 1980, 9:795-831.
Kondra et al., “Selection for Oleic, Linoleic and Linolenic Acid Content in F2 Populations of Rapeseed,”Can. J. Plant Sci., 1976, 56:961-966.
Koseoglu et al., “Chemistry, Analysis, and Nutritional Effects,” “Hydrogenation of Canola Oil,”Canola and Rapeseed: Production, Chemistry, Nutrition, and Processing Technology, 1990, Van Nostrand Reinhold, NY, Chap. 8, pp. 123-148.
Lim et al., “Expressed sequence tags of Chinese cabbage flower bud cDNA,”Plant Physiol., 1996, 111(2):577-588.
Lindqvist et al.,EMBO J., 1996, 15(16):4081-4092.
Mattson, “A changing role for dietary monounsaturated fatty acids,”J. Am. Diet. Assoc., 1989, 89:387-391.
Mensink et al., “Effect of a Diet Enriched with Monounsaturated or Polyunsaturated Fatty Acids on Levels of Low-Density and High-Density Lipoprotein Cholesterol in Healthy Women and Men,”New England J. Med., 1989, 321:436-441.
Mensink et al., “Effect of Dietary Trans Fatty Acids on High-Density and Low-Density Lipoprotein Cholesterol Levels in Healthy Subjects,”New England J. Med., 1990, 323:439-445.
Neidleman, “Temperature Effects in the Biosynthesis of Unique Fats and Fatty Acids,”Proc. of the World Conf. On Biotech. Fats Oils Ind., 1988.
Newman et al., “Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones ,”Plant Physiol., 1994, 106:1241-1255.
Okuley et al.,Plant Cell, 1994, 6:147-158.
Paul et al., “Inheritance of S-methyl-L-cysteine sulphoxide and thiocyanate contents in forage rape (Brassica napus L.),”Theor. Appl. Genet., 1986, 72:706-709.
Pleines and Friedt, “Breeding for Improved C18-Fatty Acid Composition in Rapeseed (Brassica napus L.),”Fat Sci. Technol., 1988, 90(5):167-171.
Pleines et al., “Recurrent Selection for Modified Polyenoic Fatty Acid Composition in Rapeseed (Brassica napus L.),” Abstract of The Proceedings of the 7th International Rapeseed Congress, Poznan, Poland, May 11-14, 1987.
Pleines et al., Abstract of the 43rd Lecture Meeting of Deutsche Gesellschaft fur Feltwissenschaft in Hamburg, Sep. 30-Oct. 1, 1987.
Rakow and McGregor, “Opportunities and Problems in Modification of Levels of Rapeseed C18 Unsaturated Fatty Acids,”J. Am. Oil Chem. Soc., 1973, 50:400-403.
Rakow, “Selection for Linoleic and Linolenic Acid Content in Rape Seed after Mutagenic Treatment,”Z. Pflanzenzüchtg., 1973, 69:62-82.
Ram et al., “Biotechnology for Brassica and Helianthus improvement,”Proceedings World Conference on Biotechnology for the Fats and Oils Industry, Sep. 27-Oct. 2, 1987.
Rennie et al., “Fatty Acid Composition of Oil from Soybean Seeds Grown at Extreme Temperatures,”J. Am. Oil Chem. Soc., 1989, 66:1622-1624.
Röbbelen and Nitsch, “Genetical and Physiological Investigations on Mutants for Polyenoic Fatty Acids in Rapeseed, Brassica napus L.,”Z. Pflanzenzüchtg., 1975, 75:93-105.
Röbbelen and Rakow, “Selection for Fatty Acids in Rapeseed,”Proceedings of the International Conference on the Scientific, Technology and Marketing of Rapeseed and Rapeseed Products, Sep. 20-23, 1970.
Röbbelen, “Changes and Limitations of Breeding for Improved Polyenoic Fatty Acids Content in Rapeseed,”Biotechnology for the Oils and Fats Industry, 1984, American

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Fatty acid desaturases and mutant sequences thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fatty acid desaturases and mutant sequences thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fatty acid desaturases and mutant sequences thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4076227

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.