Fast photochemical method of labelling nucleic acids for detecti

Details Fast photochemical method of labelling nucleic acids for detecti Fast photochemical method of labelling nucleic acids for detecti

1984-05-18

1988-04-12

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 935 77, 935 78, 536 27, C12Q 168, C12Q 100, C07H 1700, C12N 1500

Patent

active

047374543

ABSTRACT:
A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a .beta.-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hybridize.

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