Fast photochemical method of labelling nucleic acids for detecti

Details Fast photochemical method of labelling nucleic acids for detecti Fast photochemical method of labelling nucleic acids for detecti

1987-10-09

1990-09-25

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 7, 435 18, 435 28, 435188, 436 63, 436 94, 436501, 436504, 536 27, 546109, 548303, 935 78, C12Q 168, C07H 2100, C07D49304, G01N 3353

Patent

active

049593099

ABSTRACT:
A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a .beta.-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hydridize.

REFERENCES:
patent: 4124598 (1978-11-01), Hearst et al.
patent: 4218539 (1980-08-01), Weltman et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4582789 (1986-04-01), Sheldon et al.
patent: 4599303 (1986-07-01), Yabusaki et al.
patent: 4617261 (1986-10-01), Sheldon et al.
patent: 4737454 (1988-04-01), Dattagupta et al.
patent: 4794073 (1988-12-01), Dattagupta et al.
Zeitschrift fur Naturforschung, vol. 27b, 1972, Tubingen S. Marciani, M. Terbojevic, F. Dall'Acqua "Light Scattering and Flow Dichroism Studies on DNA After the Photoreaction with Psoralen"-pp. 196-200.
Molecular & General Genetics, vol. 179, No. 1, 1980, G. Venema, U. Canosi, "The Effect of Trimethylpsoralen-Crosslinking on Entry of Donor DNA in Transformation and Transfection of Bacillus Subtilis" pp. 1-11.
Singer-Sam, J. et al, Proc. Natl. Acad. Sci., vol. 80, pp. 802-806, 1983.
Chatterjee et al, The Journal of Biological Chemistry, vol. 257, No. 15, Aug. 10, 1982, pp. 9173-9180.
Song, et al, Annals of New York Academy of Sciences, vol. 346, pp. 355-367, 1980.
Cantor, C. R., Annals at New York Academy Sciences, vol. 346, pp. 379-385, 1980.
Schwartz et al, Cold Spring Harbor Simposia in Quantitative Biology, vol. XLVII, pp. 189-195, 1982.
Saffran; W. et al, Proc. Natl. Acad. Scien., vol. 79, pp. 4594-4598, Aug., 1982.
Transmission Electron Microscopic Method for Gene Mapping on Polytene Chromosomes by in situ Hybridization, Proc. Natl. Acad. Sci., U.S.A., vol. 78, No. 11, pp. 7059-7063, Nov. 1971, Genetics.
Site-Directed Psoralen Crosslinking of DNA, Proc. Natl. Acad. Sci. U.S.A., vol. 79, pp. 4594-4598, Aug. 1982, Wilma A. Saffran, Merrill Goldenberg and Charles R. Cantor.

Affiliated with

Also associated with

Rating

Fast photochemical method of labelling nucleic acids for detecti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fast photochemical method of labelling nucleic acids for detecti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fast photochemical method of labelling nucleic acids for detecti will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-328778