Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2006-03-08
2010-10-26
Leavitt, Maria (Department: 1633)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S471000, C435S476000
Reexamination Certificate
active
07820443
ABSTRACT:
The invention relates to a fast method of transforming competent cells, comprising:a. mixing a plasmid DNA with the competent cells suspending within ionic solution to form a mixture;b. plating the mixture on a warm selective medium; andc. culturing the mixture on the medium;wherein the ionic solution comprises divalent cation selected from the group consisting of Ca2+, Mg2+, Mn2+, Zn2+, Cu2+, Cd2+, Fe2+, Sr2+and Co2+, and provided that the ionic solution does not include Ca2+alone.
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King Anthony
Leavitt Maria
WPAT, P.C.
Yeastern Biotech Co., Ltd.
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