Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2005-03-08
2005-03-08
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S471000, C435S476000
Reexamination Certificate
active
06864088
ABSTRACT:
A fast method of transforming competent cells is described. The competent cells are thawed at room temperature or in a water bath. Plasmid DNAs and competent cells are mixed together, then the mixture is subject to heat shock treatment. After plating the mixture on a low-temperature selective medium by a low-temperature plating tool, the competent cells are cultured on the selective medium.
REFERENCES:
patent: 0073414 (2000-05-01), None
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Chen Tzu-Chih
Hua Wei-Ni
Ketter James
Thomas Kayden Horstemeyer & Risley
Vogel Nancy T.
Yeastern Biotech Co., Ltd.
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