Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-05-19
1999-03-02
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 72, 435 721, 436501, C12Q 168, G01N 3353
Patent
active
058769390
ABSTRACT:
The present invention provides mammalian protein tyrosine phosphatases, human PTP-BAS type 4, human PTP-BAS type 5a and mouse PTP-BAS type 5b, each of which is a Fas-associated protein (FAP), nucleic acid molecules encoding a PTP-BAS type 4 or a PTP-BAS type 5 and antibodies specific for a PTP-BAS type 4 or for a PTP-BAS type 5. The invention also provides methods for identifying FAP's, which can associate with Fas and can modulate apoptosis. The invention also provides screening assays for identifying an agent that can effectively alter the association of a FAP with Fas and, therefore, can increase or decrease the level of apoptosis in a cell. The invention further provides methods of modulating apoptosis in a cell by introducing into the cell a nucleic acid molecule encoding a PTP-BAS or fragment of a PTP-BAS or an antisense nucleotide sequence, which is complementary to a portion of a nucleic acid molecule encoding a PTP-BAS. The invention also provides a method of using a reagent that can specifically bind to a FAP to diagnose a pathology that is characterized by an increased or decreased level of apoptosis in a cell. The invention also provides methods of modulating apoptosis in a cell by contacting the cell with an agent that effectively alters the association of a FAP and Fas in a cell or alters the activity of a FAP in a cell.
REFERENCES:
patent: 5674734 (1997-10-01), Leder et al.
patent: 5750653 (1998-05-01), Chu et al.
Itoh et al., The Polypeptide Encoded by the cDNA for Human Cell Surface Antigen Fas Can Mediate Apoptosis, Cell 66:233-43 (1991).
Maekawa et al., "Molecular Cloning of a Novel Protein-tyrosine Phosphatase Containing a Membrane-binding Domain and GLGF Repeats," FEBS Lett. 337:200-206 (1994).
Sugawara et al., "Suppression of Stress Protein GRP78 Introduction in Tumor B/C10ME Eliminates Resistance to Cell Mediated Cytotoxicity," Cancer Res. 53:6001-6005 (1993).
Sato et al., "FAP-1: A Protein Tyrosine Phosphatase That Associates with Fas," Science 268:411-415 (1995).
Yanagisawa et al., "The Molecular Interaction of Fas and FAP-1," J. Biol. Chem. 272:8539-8545 (1997).
Takahashi and Kataoka, "Development of Anticancer Drugs Targeted of Fas-mediated Apoptosis Signal," Chem. Abstr. 126:7 (180671w) (1997).
Zhang and Winoto, "A mouse Fas-associated Protein with Homology to the Human Mort1/FADD Protein is Essential for Fas-Induced Apoptosis," Mol. Cell. Biol. 16:2756-2763 (1996).
Kalpana and Goff, "Genetic Analysis of Homomeric Interactions of Human Immunodeficiency Virus Type 1 Integrase Using the Yeast Two-hybrid System," Proc. Natl. Acad. Sci. USA. 90:10593-10597 (1993).
Holt et al., "Phospatidylinositol 3-Kinase Activation is Mediated by High-Affinity Interactions between Distinct Domains within the p110 and p85 Subunits," Mol. Cell. Biol. 14:42-49 (1994).
Keller and Manak, DNA Probes, Stockton Press, pp. 5-6 (1993).
Innis et al., PCR Protocols: Methods and Applications, Academic Press, pp. 9-10 (1990).
Suda and Nagata, "Purification and Characterization of the Fas-ligand that Induces Apoptosis," J. Exp. Med. 179:873-879 (1994).
Fields and Sternglanz, "The two-hybrid System: An Assay for Protein-Protein Interactions," Trends. Genet. 10:286-292 (1994).
Schulze-Osthoff et al., "Divergent Signalling via APO-1/Fas and the TNF Receptor, two Homologous Molecules Involved in Physiological Cell Death," EMBO J. 13:4587-4596 (1994).
Songyang et al., "Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains," Science 275:73-77 (1997).
Itoh and Nagata, "A Novel Protein Domain Required for Apoptosis," J. Biol. Chemistry 268:10932-10937 (1993).
Chinnaiyan et al., Cell 81, 505-512, 1995.
Tartaglia et al., Cell 74, 845-853, 1993.
Reed John C.
Sato Takaaki
Horlick Kenneth R.
La Jolla Cancer Research Foundation
LandOfFree
FAS associated proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with FAS associated proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and FAS associated proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-421369