Family of proteins belonging to the pancreatic ribonuclease...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C530S324000, C530S395000, C514S012200, C514S021800, C435S199000

Reexamination Certificate

active

06239257

ABSTRACT:

BACKGROUND OF THE INVENTION
The invention relates to pharmaceuticals, and more particularly relates to pharmaceuticals for use in treating tumors in humans.
One object of the invention is to provide a family of pharmaceuticals for use in treating tumors in humans.
Another object is, in general, to improve upon known therapies for treatment of tumors in humans.
In accordance with the invention, there is a provided a family of highly-homologous and closely related proteins. The family is exemplified by four purified proteins, each having a) an amino acid sequence that is 114 amino acids long, b) an isoelectric point of approximately 10, and c) a molecular weight of approximately 13,000. Advantageously although not necessarily, the proteins are derived from frog eggs and preferably from the eggs of the
Rana pipiens
frog.
The above-referenced four purified proteins are glycoproteins, i.e. each contains glycans (carbohydrates) that are linked to the protein by covalent bonds. However, the glycan moieties are not necessary for the bioactivity of the proteins against human tumor cell lines.
U.S. Pat. Nos. 5,559,212 and 5,728,805 disclose two protein peaks coming off a cationic-exchange chromatography column. U.S. Pat. No. 5,559,212 describes the amino acid sequence and composition of the protein in the first (larger) peak, and the disclosure of U.S. Pat. No. 5,728,805 extends this description by describing the amino acid sequence and composition of the protein in the second (smaller) peak. A third protein peak also comes off the ion-exchange column, and this (still smaller) peak has now been shown to contain four proteins that are sufficiently similar to justify their designation as a family. Because the third protein peak is eluted after the first and second peaks, the proteins in that peak are more basic.
Tests have shown that each of the four proteins possesses bioactivity against two human carcinoma cell lines.


REFERENCES:
patent: 4882421 (1989-11-01), Shogen et al.
patent: 5559212 (1996-09-01), Ardelt
patent: 5728805 (1998-03-01), Ardelt
Kamiya et al.,J. Biochem.,vol. 108, pp. 139-143, 1990.*
Ardell et al.,The Journal of Biological Chemistry,vol. 266, No. 1, pp. 245-251, Jan. 5, 1991.*
Mosimann et al.,Protein: Structure and Genetics,vol. 14, pp. 392-400, 1992.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Family of proteins belonging to the pancreatic ribonuclease... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Family of proteins belonging to the pancreatic ribonuclease..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Family of proteins belonging to the pancreatic ribonuclease... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2484930

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.