Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-06-05
1999-02-16
Low, Christopher S. F.
Chemistry: molecular biology and microbiology
Vector, per se
435 601, 435311, 4351721, 4351723, 435194, 435325, 435375, 435377, 4352523, 4352542, 800 2, 536 232, 536 235, C12N 1563
Patent
active
058720065
ABSTRACT:
The present invention relates to a newly identified family of protein serine/threonine kinases which phosphorylate microtubule-associated protein 2 (MAP2). It is based, in part, on the cloning and characterization of novel MAP2 kinases designated extracellular signal-regulated kinase 1, 2, and 3 (ERK1, ERK2, ERK3) which are expressed in the central nervous system, and on the identification of another ERK family member, ERK4, with antisera. The present invention provides for recombinant nucleic acid molecules and proteins representing members of the MAP2 kinase family, and also for microorganisms, transgenic animals, and cell lines comprising recombinant MAP2 kinase molecules. In additional embodiments of the invention, the present invention provides for methods for assaying cellular factor activity, including, but not limited to, nerve growth factor activity, in which the activation of MAP2 kinase serves as an indicator of cellular factor activity. These methods may be extremely useful in screening compounds for the presence of a desired cellular factor activity. In specific embodiments, compounds which may be useful in the treatment of Alzheimer's disease, peripheral neuropathies, and diabetes may be identified using the methods of the invention.
REFERENCES:
patent: 4599305 (1986-07-01), Witte et al.
A. Lorincz et al. Nature, 307:183-185, Jan. 12, 1984.
Loeb, et al., (1992) Neuron 9: 1053-1065.
Makowski, et al., (1988) J. Biol. Chem. 7: 3402-3410.
Boulton, et al., (1991) Cell 65: 663-675.
Sanghera, et al., (1991) J. Biol. Chem. 266: 6700-6707.
Boulton and Cobb, (1991) Cell Regul. 2: 357-371.
Ely, et al., (1990) J. Cell Biol. 110: 731-742.
Anderson, et al., (1990) Nature 343: 651-653.
Boulton, et al., (1990) Science 249: 64-67.
Boulton, et al., (1990) J. Biol. Chem. 265: 2713-2719.
Gregory, et al., (1989) J. Biol. Chem. 264: 18397-18401.
Rossomando, et al., (1989) Proc. Natl. Acad. Sci. U.S.A. 86: 6940-6943.
Liou, (1989) J. Biol. Chem. 264: 8200-8206.
Cicirelli, (1988) J. Biol. Chem. 263: 2009-2019.
Sturgill, et al., (1988) Nature 334: 715-718.
Ray and Sturgill, (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1502-1506.
Hanks, et al., (1988) Science 241: 42-52.
Courchesne, et al., (1989) Cell 58: 1107-1119.
Elion, et al., (1990) Cell 60: 649-664.
Ray, et al., (1988) PNAS 85: 3753-3757.
R. Aebersold, et al., (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 6970-6974.
K. Matsushima, et al., (1988) J. Exp. Med 107: 1883-1893.
E. Blasi, et al., (1985) Nature 318: 667-670.
M. Jaye, et al., (1983) Nucleic Acids Research 11: 2325-2335.
L.B. Ray, et al., (1988) J. Biol. Chem 263: 12721-12727.
H. Land, et al., (1983) Nature 304: 596-605.
J.W. Gordon, et al., (1981) Science 214: 1244-1246.
A. Lorincz, et al. (1984), Nature, 307: 183-185.
Boulton Teri G.
Cobb Melanie H.
Nye Steven
Panayotatos Nikos
Yancopoulos George D.
Board of Regents , The University of Texas System
Cobert Robert J.
Low Christopher S. F.
Nguyen Dave Trong
Regeneron Pharmaceuticals Inc.
LandOfFree
Family of MAP2 protein kinases does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Family of MAP2 protein kinases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Family of MAP2 protein kinases will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2061633