Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase...

Details Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase... Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase...

2011-07-12

2011-07-12

McElwain, Elizabeth F (Department: 1638)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S440000, C435S320100, C435S419000, C435S252300

Reexamination Certificate

active

07977469

ABSTRACT:
The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.

REFERENCES:
patent: 5246842 (1993-09-01), O'Brien et al.
patent: 5614393 (1997-03-01), Thomas et al.
patent: 5968809 (1999-10-01), Knutzon et al.
patent: 5972664 (1999-10-01), Knutzon et al.
patent: 6051754 (2000-04-01), Knutzon
patent: 6897050 (2005-05-01), Napier
patent: 7531347 (2009-05-01), Knutzon et al.
patent: 2363064 (2002-04-01), None
patent: WO-9306712 (1993-04-01), None
patent: WO-9621022 (1996-07-01), None
patent: WO-9803671 (1998-01-01), None
patent: WO-9846763 (1998-10-01), None
patent: WO-9846764 (1998-10-01), None
patent: WO-9846765 (1998-10-01), None
patent: WO-9927111 (1999-06-01), None
patent: WO-9933958 (1999-07-01), None
patent: WO-99/64614 (1999-12-01), None
patent: WO-0012720 (2000-03-01), None
patent: WO-0020602 (2000-04-01), None
patent: WO-0020603 (2000-04-01), None
patent: WO-0021524 (2000-04-01), None
patent: WO-0021557 (2000-04-01), None
patent: WO-0034439 (2000-06-01), None
patent: WO-00/40705 (2000-07-01), None
patent: WO-0051444 (2000-09-01), None
patent: WO-0075341 (2000-12-01), None
patent: WO-02081668 (2002-10-01), None
patent: WO-03012092 (2003-02-01), None
patent: WO-03078639 (2003-09-01), None
patent: WO-2004090123 (2004-10-01), None
Broun et al, Science 282: 1315-1317, Nov. 13, 1998.
Van de Loo et al, PNAS, USA 92: 6743-6747, Jul. 1995.
Doerks et al, TIG 14(6): 248-250, Jun. 1998.
Brenner, S.E., TIG 15(4): 132-133, Apr. 1999.
Bork et al, TIG 12(10): 425-427, Oct. 1996.
Sequence alignment of SEQ ID No. 3 with US Patent 7,531,347, SEQ ID No. 23, Mukerji et al, alignment dated Jun. 21, 2010.
Los, D. A., et al., “Structure and Expression of Fatty Acid Desaturases”, Biochimica et Biophysica Acta, 1998, vol. 1394, pp. 3-15.
Communication Pursuant to Rule 62 EPC including Extended European Search Report dated Apr. 3, 2010 Issued in European Application No. 10150355.5.
Bork, P., et al., “Go Hunting in Sequence Databases but Watch Out for Traps”, TIG, 1996, vol. 12, No. 10, pp. 425-427.
Brenner, S. E., “Errors in Genome Annotation”, TIG, 1996, vol. 15, No. 4, pp. 132-133.
Broun, P., et al., “Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids”, Science, 1998, vol. 282, pp. 1315-1317.
De Luca, V., “Molecular Characterization of Secondary Metabolic Pathways”, AgBiotech News and Information, 1993, vol. 5, No. 6 pp. 225N-229N.
Doerks, T., et al., “Protein Annotation: Detective Work for Function Prediction”, TIC, 1998, vol. 14, No. 6, pp. 248-250.
Girke, T., et al., “Identification of a Novel 6-acyl-group Desaturase by Targeted Gene Disruption inPhyscomitrella patens”, The Plant Journal, 1998, vol. 15, No. 1, pp. 39-48.
Hong, H., et al., “High-Level Production of -Linolenic Acid inBrassica junceaUsing a 6 Desaturase fromPythium irregulare”, Plant Physiology, 2002, vol. 129, pp. 354-362.
Knutzon, D. S., et al., “Identification of 5-Desaturase fromMortierella alpinaby Heterologous Expression in Bakers' Yeast and Canola”, The Journal of Biology Chemistry, 1998, vol. 273, No. 45, pp. 29360-29366.
Michaelson, L. V, et al., “Functional Identification of a Fatty Acid 5-Desaturase gene fromCaenorhabditis elegans”, FEBS, 1998, vol. 439, pp. 215-218.
Michaelson, L. V., et al., “Isolation of a5-Fatty Acid Desaturase Gene fromMortierella alpina”, The Journal of Biological Chemistry, 1998, vol. 273, No. 30, pp. 19055-19059.
Napier, J. A., et al., “Identification of aCaenorhabditis elegans6-fatty-acid-desaturase by Heterologous Expression inSaccharomyces cerevisiae”, Biochem. J., 1998, vol. 330, pp. 611-614.
Qiu, X., et al., “Identification of a 4 Fatty Acid Desaturase fromThraustochytriumsp. Involved in the Biosynthesis of Docosahexanoic Acid by Heterologous Expression inSaccharomyces cerevisiaeandBrassica juncea”, The Journal of Biological Chemistry, 2001, vol. 276, No. 34, pp. 31561-31566.
Saito, T., et al., “Identification of a5-Fatty Acid Desaturase from the Cellular Slime MoldDictyostelium discoideum”, Eur. J. Biochem., 1999, vol. 265, pp. 809-814.
Sayanova, O., et al., “Expression of a Borage Desaturase cDNA Containing an N-Terminal Cytochromeb5Domain Results in the Accumulation of High Levels of6-Desaturated Fatty Acids in Transgenic Tobacco”, Proc. Natl. Acad. Sci., 1997, vol. 94, pp. 4211-4216.
Smith, T. F., et al., “The Challenges of Genome Sequence Annotation or, ‘The Devil is in the Details’”, Nature Biotechnology, 1997, vol. 15, pp. 1222-1223.
Sperling, P., et al., “A Bifunctional 6-Fatty Acyl Acetylenase/Desaturase from the mossCeratodon purpureus”, Eur. J. Biochem., 2000, vol. 267, pp. 3801-3811.
Van De Loo, F. J., et al., “An Oleate 12 Hydroxylase fromRicinus communisL. is a Fatty Acyl Desaturase Homolog”, PNAS USA, 1995, vol. 92, pp. 6743-6747.
Watts, J. L., et al., “Isolation and Characterization of a5-Fatty Acid Desaturase fromCaenorhabditis elegans”, Biochemistry and Biophysics, 1999, vol. 362, No. 1, pp. 175-182.
Hong, H., et al., “Isolation and Characterization of a5FA Desaturase fromPythium irregulareby Heterologous Expression inSaccharomyces cerevisiaeand Oilseed Crops”, Lipids, 2002, vol. 37, No. 9, pp. 863-868.
Huang, Y.-S., et al., “Cloning of12and6-Desaturases fromMortierella alpinaand Recombinant Production of -Linolenic Acid inSaccharomyces cerevisiae”, Lipids, 1999, vol. 34, No. 7, pp. 649-659.
Sakuradani, E., et al., “6-Fatty Acid Desaturase from an Arachidonic Acid-ProducingMortierellaFungus, Gene Cloning and its Heterologous Expression in a Fungus,Aspergillus”, Gene, 1999, vol. 238, pp. 445-453.
Sambrook, J., et al., “Molecular Cloning, a Laboratory Manual”, Second Edition, Cold Spring Harbor Laboratory Press, 1989, pp. 11.4 and 14.15.
Wu, G., et al., “Stepwise Engineering to Produce High Yields of Very Long-Chain polyunsaturated Fatty Acids in Plants”, Nature Biotechnology, 2005, vol. 23, No. 8, pp. 1013-1017.
Partial European Search Report for Application No. 07019867.6-1212, dated Mar. 31, 2008.
Saito, T., et al., “A Second Functional Δ5 Fatty Acid Desaturase in the Cellular Slime MouldDictyostelium discoideum”, Eur. J. Biochem., 2000, vol. 267, pp. 1813-1818.
Notice of Reasons for Rejection dated Jul. 29, 2009 issued in JP Appl. No. 2009-076067 and the English translation thereof.
O'Brien, D. J., et al., “Production of Eicosapentaenoic Acid by the Filamentous FungusPythium irregulare”, Applied Microbiology and Biotechnology, 1993, vol. 40, pp. 211-214.
Pankhurst, C. E., et al., “The Usefulness of Fatty Acid Analysis in Differentiating Species ofPythium, RhizoctoniaandGaeumannomycesand as a Tool for their Detection in Infected Wheat Roots”, Australasian Plant Pathology, 2001, vol. 30, pp. 191-197.
Communication Pursuant to Article 94(3) EPC dated Aug. 24, 2009 issued in European Application No. 07019867.6.

Affiliated with

Also associated with

Rating

Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2715105