Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1980-11-03
1982-03-09
Wiseman, Thomas
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435810, 23230B, 424 12, G01N 3354, C12N 996
Patent
active
043189830
ABSTRACT:
A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.
REFERENCES:
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patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4230797 (1980-10-01), Boguslaski et al.
patent: 4235869 (1980-11-01), Schwarzberg
patent: 4238565 (1980-12-01), Hornby et al.
Hornby William E.
Morris David L.
Klawitter Andrew L.
Miles Laboratories Inc.
Wiseman Thomas
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