Factor VIII concentrates prepared from heparinized plasma by the

Details Factor VIII concentrates prepared from heparinized plasma by the Factor VIII concentrates prepared from heparinized plasma by the

1981-11-02

1983-05-17

Rosen, Sam

Mechanical guns and projectors

Projectile impelled by user-provided force

424 94, 424177, A61K 3514

Patent

active

043839894

ABSTRACT:
A method for recovering Antihemophilic Factor VIII from whole blood or blood plasma or blood plasma fractions freshly collected directly into an anticoagulant selected from the group of heparin, sodium heparin, or mixtures thereof, the heparin being used in the range of 6-8 units/ml of plasma or 3.5-4 units/ml of blood; and either first separating the red cells from the plasma and/or freezing the heparinized plasma, resolubilizing the plasma, isolating a cryoprecipitate from the plasma, resolubilizing the cryoprecipitate, adding a saline heparin solution to the resolubilized cryoprecipitate, incubating the resolubilized cryoprecipitate at a temperature of from about 0.degree. C. to about 4.degree. C. for a time of at least 2 hours, whereby Factor VIII present in the cryoprecipitate is insolubilized using heparin precipitable cold insoluble globulin and the resulting Factor VIII rich precipitate also includes cold insoluble globulin, separating the Factor VIII rich precipitate, and isolating Factor VIII therefrom. This new process permits recovery of a high purity Factor VIII preparation which can easily be made in the blood bank or alternatively, the cold precipitation step can be carried out in a fractionation plant.

REFERENCES:
patent: Re29698 (1978-07-01), Fekete et al.
patent: 3803115 (1974-04-01), Fekete et al.
patent: 4203891 (1980-05-01), Rock
patent: 4210580 (1980-07-01), Amrani
patent: 4289691 (1981-09-01), Rock et al.

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