Factor VIII:Ca chromogenic assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor

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435217, 435219, C12Q 156

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active

061000507

ABSTRACT:
A chromogenic assay for determination of blood coagulation Factor VIII:Ca, using an indicator of Factor Xa simultaneously as a measure of Factor VIII:Ca concentration and as an inhibitor of Factor Xa. This technique can be applied to measure the concentration of an activating enzyme using the rate of conversion of the indicator molecule of the product of that enzyme as an indirect indicator of enzyme concentration.

REFERENCES:
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patent: 5506112 (1996-04-01), Lang et al.
Wagenvoord R., Development of a Simple Chromogenic Factor VIII Assay . . . Haemostasis 1989 19: 196-204.
Pieters J., In Situ Generated Thrombin is the Only Enzyme . . . Blood vol. 74 No. 3 1989 pp. 1021-1024.
Wagenvoord R., Development of a Sensitive & Rapid Chromogenic Factor IX Assay . . . Haemostasis 1990: 20 276-288.
Van Dieijen G., Assembly of the Intrinsic Factor X Activating Complex . . . Thromb Haemo 53 (3) 396-400 1985.
Kessels H., A Method For Measuring Activated Factor VIII in Plasma, Thromb Haemost 66 (4) 430-434 1991.
Wagenvoord, R., Hendrix H., Hemker, H., Development of a Simple Factor VIII Assay for Clinical Use, (Abstract), Thrombosis Haemostasis; 58:p.341 (1987).
Scandinavian Journal of Haemotology, Supplement No. 40, vol. 33 (1984)(Copenhaen, DK) Rosen: "Assay of factor VIII:C with a chromogenic substrate", pp. 139-145.
Haemostasis, vol. 17, No. -2, Jan.-Apr. 1987, Karger AG, (Basel, CH), G. Van Dieijen et al.: "Spectrophotometric Method for the assay of human blood coagulation factor VIII", pp. 14-24.
Biochemistry, vol. 19 No. 3, Feb. 5, 1980, American Chemical Society, (Washington, US), G.A. Vehar et al.: "Preparation and properties of bovine factor VIII (antihemophilic factor)", pp. 401-410.
Biochem. vol. 11 (1972) Fujikawa et al.: Bovine Factor XI (Stuart Factor) Mechanism of activation by Russel's Viper Venom, 4892.

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