Factor IX production in transgenic non-human mammals and factor

Details Factor IX production in transgenic non-human mammals and factor Factor IX production in transgenic non-human mammals and factor

1996-11-01

2000-04-04

Crouch, Deborah

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

Transgenic nonhuman animal

800 7, 435 696, 435212, 536 232, 536 235, A01K 67027, C12P 2100, C12P 2104, C07H 2104

Patent

active

060463805

ABSTRACT:
Poor expression yields of recombinant human factor IX are attributable to aberrant splicing in heterologous expression systems such as transgenic hosts for example, transgenic nonhuman mammal The aberrant splicing sites have been identified as (a) a donor site including mRNA nucleotide 1085; and (b) an acceptor site including mRNA nucleotide 1547; adopting the mRNA nucleotide numbering of FIG. 2 of the drawings. Improved factor IX expression sequences have at least one of these sites engineered out, so as to prevent or reduce the effect of aberrant splicing and to increase yields. The improved DNA sequences may also be useful in gene therapy.

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