Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1998-08-17
2000-07-18
Prouty, Rebecca E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C07K 100, C07H 2102
Patent
active
060909196
ABSTRACT:
Three classes of GFP mutants having single excitation maxima around 488 nm are brighter than wild-type GFP following 488 nm excitation. GFPmut1 has a double substitution: F64L, S65T; GFPmut2 has a triple substitution: S65A, V68L, S72A; and GFPmut3 is characterized by the double substitution S65G, S72A. The excitation maxima of the three mutants are at 488 nm, 481 nm and 501 nm respectively. The fluorescence intensities following excitation at 488 nm are an order of magnitude higher than that of wild-type GFP excited at 488 nm in E. coli. The expression of GFP is observable minutes after induction.
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Cormack Brendan P.
Falkow Stanley
Valdivia Raphael H.
Prouty Rebecca E.
Sherwood Pamela
Stole Einar
The Board of Trustees of the Leland Stanford Junior University
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